Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Alkaloid Casuarinine H and use thereof in preparation of medicines for treating neurodegenerative diseases

A neurodegenerative and alkaloid technology, applied in nervous system diseases, neuromuscular system diseases, drug combinations, etc., can solve problems such as drugs for neurodegenerative diseases that have not yet been seen, and achieve rich natural resources, wide distribution and strong regeneration ability. Effect

Active Publication Date: 2014-08-13
FUDAN UNIV
View PDF3 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there have been no reports on the use of the alkaloid Casuarinine H in the extract of vine stone pine in the preparation of drugs for the treatment of neurodegenerative diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alkaloid Casuarinine H and use thereof in preparation of medicines for treating neurodegenerative diseases
  • Alkaloid Casuarinine H and use thereof in preparation of medicines for treating neurodegenerative diseases
  • Alkaloid Casuarinine H and use thereof in preparation of medicines for treating neurodegenerative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1 kg of the whole plant of Fujishipine, crushed, and cold-soaked with 3 times the amount of 90% methanol at room temperature for 24 hours, a total of 3 times. The extracts were combined three times and concentrated under reduced pressure to obtain 90 g of extract. The extract was suspended with 2 times the amount of 3% tartaric acid aqueous solution, extracted three times with ethyl acetate equal to the volume of the suspension, and then washed with Na 2 CO 3 Adjust the tartaric acid aqueous solution to pH = 10, then extract three times with chloroform, and concentrate the chloroform extraction under reduced pressure to obtain 1.1 g of total alkaloid extract, then mix the sample with equal-fold silica gel (100-200 mesh), and use 50 times Silica gel (200-300 mesh) was used for column chromatography, and dichloromethane: methanol was used as the mobile phase gradient elution (volume ratio 1:0-0:1). Collect dichloromethane:methanol (15:1, volume ratio) eluted fractions, ...

Embodiment 2

[0020]1 kg of the whole herb of Fujishipine was crushed, refluxed with 80% ethanol at room temperature for 3 hours, repeated 3 times, the extracts were combined, concentrated under reduced pressure to obtain 110 g of extract. The extract was suspended with 2 times the amount of 3% tartaric acid aqueous solution, extracted three times with ethyl acetate equal to the volume of the suspension, and then washed with Na 2 CO 3 The tartaric acid aqueous solution was adjusted to pH = 10, and extracted three times with an equal volume of chloroform. The chloroform extraction part was concentrated under reduced pressure to obtain 1.3 g of total alkaloid extract. The macroporous resin HP-20 on the extract was eluted with water, 50% ethanol, and 90% ethanol respectively, and the eluted part of 50% ethanol was applied to Sephadex LH-20, which was eluted with dichloromethane:methanol 2:1, and passed TLC detection combined to obtain 13 mg of Casuarinine H.

Embodiment 3

[0021] The neuroprotective effect test of embodiment 3 Casuarinine H

[0022] SHSY-5Y cells were digested and suspended in MEM / F12 medium containing 10% fetal bovine serum. Take 2×10 5 SHSY-5Y cells were seeded on 96-well culture plates at a density of 1 / ml, and the inoculation volume was 100 μl / well, and then placed in 5% CO 2 Incubate in a constant temperature incubator at 37°C for 24 hours. Replace the culture solution in each well with fresh MEM / F12 culture solution, add the corresponding concentration of the compound to be tested (10 microliters / well) in the administration group, normal control group and H 2 o 2 The compound solvent control (10 μl / well) was added to the injury group. After incubation for 2 hours, in the administration group with H 2 o 2 1mM H 2 o 2 (10 μl / well), the final concentration is 100 μM. After continuing to culture for 24 hours, add 5 mg / mL MTT (10 μl / well) for live cell staining. After incubation for 3 hours, discard the culture medium...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of medicine technology, relates to lycopodiastrum casuarinoides extract alkaloid Casuarinine H and medicinal new use thereof, and in particular relates to use of the lycopodiastrum casuarinoides extract alkaloid Casuarinine H in preparation of medicines for treating neurodegenerative diseases. Activity study on the lycopodiastrum casuarinoides extract alkaloid Casuarinine H is performed by an oxidative stress in-vitro screening model, and experiments show that the alkaloid Casuarinine H has a an obvious protective effect on cell injury caused by oxidative stress, the alkaloid Casuarinine H can be used alone or in combination, or in combination with suitable excipients, oral or non oral dosage preparation forms such as tablets, capsules, granules or injections are produced according to conventional methods, and the alkaloid Casuarinine H can be used for preparing the medicines for treating the neurodegenerative diseases including but not limited to Alzheimer's disease or Parkinson's disease and the like.

Description

technical field [0001] The present invention relates to the technical field of medicine, and relates to the alkaloid Casuarinine H in the extract of rattan stone pine and its new application in medicine, in particular to the use of the alkaloid Casuarinine H in the extract of rattan rock pine in the preparation of medicines for treating neurodegenerative diseases, and in particular to rattan rock pine The use of the alkaloid Casuarinine H in the extract in the preparation of drugs for the treatment of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis. The present invention also relates to the extraction and preparation of Casuarinine H from vine stone pine method. Background technique [0002] Studies have shown that neurodegenerative diseases such as Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis (ALS), Huntington's disease (Huntington's disease) sdisease, HD), etc. not only ser...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D221/22A61K31/4748A61P25/28A61P25/16A61P21/00
CPCC07D221/22
Inventor 胡金锋杨国勋唐宇熊娟胡长玲
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com