Unlock instant, AI-driven research and patent intelligence for your innovation.

Oligopeptide for diagnosing and treating hepatitis B and application of oligopeptide

A technology for hepatitis B and short peptides, applied in the field of short peptides and their modified labeling series, can solve the problems of limiting the research progress of HBV infection mechanism, and achieve the effect of simple structure and short sequence

Inactive Publication Date: 2014-08-13
CHONGQING ACADEMY OF SCI & TECH
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, due to the particularity of HBV genome structure, there are still many difficulties in labeling HBV through gene recombination technology, which largely limits the research progress of HBV infection mechanism

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oligopeptide for diagnosing and treating hepatitis B and application of oligopeptide
  • Oligopeptide for diagnosing and treating hepatitis B and application of oligopeptide
  • Oligopeptide for diagnosing and treating hepatitis B and application of oligopeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Synthesized peptide mixture T20 (synthesized by Shanghai Jier Biochemical Co., Ltd.), and verified by ELISA method;

[0020] ① Coat the ELISA reaction plate with polypeptide mixture T20 according to the concentration of 40 μg / ml, coat 100 μl per well, incubate overnight at 4°C, take the uncoated polypeptide well as the blank control, and take the HepG2 cell supernatant without HBV particles as negative As a control, the HepG2.2.15 supernatant containing HBV particles (the content of HBV particles was 1×10 7 UI / ml) is the detection group, and three replicate wells are set for each group; ② Discard the coating solution, wash the plate twice with 0.05% (v / v) PBST buffer, 5 minutes each time; ③ Discard the washing solution , add 5% (m / v) BSA solution to each well, and block for 1 hour at 4°C; ④ Discard the blocking solution, wash the plate 3 times with 0.05% (v / v) PBST buffer, 5 minutes each time; ⑤ Discard the washing Plate solution, add 100 μl 0.05% (v / v) PBST buffer ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an oligopeptide for diagnosing and treating hepatitis B and an application of the oligopeptide. The amino acid sequence of the oligopeptide is alanine-alanine-alanine-alanine. The oligopeptide has a high combining capacity with hepatitis B virus (HBV), and a linear serial set formed by a modified marker (in linear connection in series with 7 arginine and green fluorescent marks) remarkably inhibit duplication and proliferation of HBV in hepatic cells. The oligopeptide is short in sequence and simple in structure and is suitable for industrialized production on a large scale together with the modified marker.

Description

technical field [0001] The invention relates to a polypeptide drug, in particular to a short peptide that can be marked and combined with hepatitis B virus and its modified label series. Background technique [0002] Chronic hepatitis B is caused by hepatitis B virus (HepatitisBvirus, HBV) and is a major disease that is prevalent worldwide. Currently, about 400 million people in the world are infected with HBV. Chronic hepatitis B can develop with the course of the disease and deteriorate into liver cirrhosis and primary liver cancer, causing death of the patient. Although it has been reported that a variety of anti-HBV drugs (such as interferon and nucleoside analogs) can effectively inhibit virus replication, inhibit the progression of the disease or cure chronic hepatitis B, but because HBV has multiple genotypes and serotypes, the above drugs Unsatisfactory on overall therapeutic effect and cure rate. Novel anti-HBV drugs and strategies are urgently needed to cure chro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K5/103A61K38/07A61P31/20C12Q1/70C12R1/93
Inventor 张华堂王超王文举郭彦
Owner CHONGQING ACADEMY OF SCI & TECH