Closed type mixotrophic culture method for microalgae and culture system thereof

Abstract

The invention relates to microalgae culture technologies and particularly relates to a closed type mixotrophic culture method for microalgae and a culture system thereof. The method comprises the steps: adding a culture medium, sterile water and a microalgae species into a material supplementing device, and then, conveying into a photo-reactor for constant-speed-flowing culture for 7-10 days under the conditions that the light intensity is 40-100 micromole / m<2> / s, the culture temperature is 25-37 DEG C and the flowing speed is 0.2-0.6m / s; enabling an algae solution to flow into a harvesting separator from the photo-reactor, discharging 70-90% of algae solution (taken as a fresh algae solution) out of the harvesting separator through an algae solution collection opening, refluxing the rest 10-30% of algae solution (taken as the microalgae species) into the material supplementing device from an algae species charging opening through a reflux pump, simultaneously supplementing the culture medium and the sterile water, then, continuing constant-speed-flowing culture, and repeating the above culture steps round by round, thereby completing the closed continuous culture of the microalgae. The culture method disclosed by the invention has the advantages that the continuous production of the microalgae is realized, the yield of the microalgae is increased, and the production cost is reduced.

Description

technical field [0001] The invention relates to a microalgae cultivation technology, in particular to a microalgae closed-type concurrent cultivation method and a cultivation system thereof. Background technique [0002] Microalgae (microalgae) is a large class of microscopic single-celled algae that exist in the form of individuals, chains or groups, ranging in size from a few microns to hundreds of microns. There are many kinds of microalgae, it is estimated that there are about 200,000-800,000 species, of which more than 35,000 species have been recorded. Microalgae are divided into autotrophic microalgae, heterotrophic microalgae and facultative microalgae. Since the 1960s, when Japan first started commercial large-scale microalgae farming, people have been able to realize the commercial production of microalgae in various fields such as health food, pharmaceutical raw materials, beauty, and feed. [0003] There are two main methods of microalgae large-scale self-culti...

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Problems solved by technology

[0006] At present, large-scale commercial cultivation of chlorella is mainly carried out in photobioreactors, which cover a large area, have a long production cycle, and have low growth density, which increases production costs in disguise and limits large-scale chlorella cultivation. develop

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