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A method of preparing cucurbitacin B

A cucurbitacin and extraction technology, applied in the field of medicine, can solve the problems of high impurity content in the extract, complicated separation and purification process, unsuitable for large-scale production, etc., and achieve the effect of low toxicity, low dosage and low impurity content

Active Publication Date: 2014-08-20
TIANJIN INSTITUTE OF PHARMA RESEARCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods consume a large amount of solvent in one step of plant extraction, the pollution in the production process is serious, the obtained extract has high impurity content, and the subsequent separation and purification process is complicated, which is not suitable for large-scale production.

Method used

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  • A method of preparing cucurbitacin B

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 2 kg of melon pedicles, crush them, pass through a 40-mesh sieve, and place them in the extraction kettle. Set the extraction pressure to 15 MPa, the extraction temperature to 40°C, the temperature of the analysis kettle I to be 45°C, the temperature of the analysis kettle II to be room temperature, and the pressure of the analysis kettles I and II to All are system pressure, after static extraction for 15min, then dynamic extraction for 25min (frequency 35KHz), sample I is obtained from the discharge port of analysis kettle I; Set the extraction pressure at 25MPa, the extraction temperature at 45°C, the temperature of the analysis kettle I at 50°C, the temperature of the analysis kettle II at 45°C, the pressures of the analysis kettle I and II are both the system pressure, and the dynamic extraction is 30min (frequency 35KHz) from the analysis kettle I. Feed mouth to get the extract. The solvent was recovered under reduced pressure and concentrated to a thick past...

Embodiment 2

[0023] Take 2 kg of melon pedicles, crush them, pass through a 40-mesh sieve, and place them in an extraction kettle. Set the extraction pressure to 25 MPa, the extraction temperature to 45°C, the temperature of the analysis kettle I to be 50°C, the temperature of the analysis kettle II to be room temperature, and the pressure of the analysis kettles I and II to Both are system pressure, add 3 times the ethanol of medicinal material amount (V / W) from carrier agent tank, after static extraction 25min, then dynamic extraction 40min (frequency 35KHz), get total cucurbitacin extract from the discharge port of analysis kettle I .

Embodiment 3

[0025] Take 2 kg of melon pedicles, crush them, pass through a 40-mesh sieve, and place them in an extraction kettle. Set the extraction pressure to 20 MPa, the extraction temperature to 40°C, the temperature of the analysis kettle I to be 45°C, the temperature of the analysis kettle II to be room temperature, and the pressure of the analysis kettles I and II to Both are system pressure, after static extraction for 10 minutes, then dynamic extraction for 30 minutes (frequency 35KHz), sample I is obtained from the discharge port of analysis kettle I; Esters, set the extraction pressure to 30MPa, the extraction temperature to 50°C, the temperature of the analysis kettle I to 55°C, the temperature of the analysis kettle II to 50°C, the pressure of the analysis kettle I and II to be the system pressure, and the dynamic extraction for 30min (frequency 35KHz) from the analysis kettle The I outlet gets the extract. The solvent was recovered under reduced pressure and concentrated to ...

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PUM

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Abstract

The invention discloses a method of preparing a natural product cucurbitacin B. According to the method, a supercritical fluid extraction method is adopted to extract a total cucurbitacin extract product from plant, then a silica gel column separation and purification manner is adopted to obtain the cucurbitacin B, and the purity of the cucurbitacin B is higher than 90%. Compared with the prior art, the method for preparing the cucurbitacin B has advantages of short time, high efficiency, small solvent using amount, low environment pollution, and suitability for large-scale production and operation.

Description

technical field [0001] The invention belongs to the technical field of medicine and relates to a preparation method of natural product cucurbitacin B, in particular to a method for extracting and preparing cucurbitacin B from plants. Background technique [0002] Cucurbitacin B (Cucurbitacin B), synonym Cucurbitacin B, Amarin, Fabacein II, molecular formula C 32 h 46 o 8 , molecular weight 542.75. [0003] Chemical Structure [0004] [0005] Cucurbitacin B is ubiquitously present in various plants of the family Cucurbitaceae, and the cucurbitacin in the cucurbitacin preparations currently on the market is derived from the dried mature fruit stalk of the Cucurbitaceae plant melon (Cucumis melo L.). [0006] Cucurbitacin B has physiological activities such as anti-hepatitis and anti-tumor. [0007] In the prior art, extraction methods such as organic solvent reflux, percolation extraction and biological enzymes are mostly used (patent "a method for extracting and sepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00
CPCY02P20/54
Inventor 刘素香刘毅白雪陈常青
Owner TIANJIN INSTITUTE OF PHARMA RESEARCH
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