A carp liver injury model and its application

A liver injury and carp technology, applied in the field of medicine, can solve the problems of ecological balance and human health threats, restrict the research and development of fish liver protection drugs, and the economic losses of aquatic products and animal husbandry, and achieve strong repeatability and short cycle , source consistent effect

Inactive Publication Date: 2017-02-22
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] 2,3,7,8-tetrachlorodiphenyl-p-dioxin (TCDD) is a highly toxic compound, mainly derived from industrial waste and pesticide abuse, etc., it not only brings huge It also poses a serious threat to ecological balance and human health. The study of the toxicity mechanism of dioxins has become one of the current scientific research hotspots, but there are still relatively few reports on the toxicity mechanism of dioxins.
For aquatic animals, there are no reports at home and abroad on the application of fish liver fine tissue slice injury models to screen fish hepatoprotective drugs, which restricts the research and development of fish hepatoprotective drugs

Method used

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  • A carp liver injury model and its application
  • A carp liver injury model and its application
  • A carp liver injury model and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Establishment of Carp Liver Injury Model

[0017] Select the experimental carp with a weight of about 900g to anesthetize with 100mg / L MS-222, then put the anesthetized carp into the ultra-clean workbench under aseptic conditions to take the liver, and place it in the L-15 ice bath pre-saturated with oxygen. In the culture medium, a liver tissue block of about 4mm×4mm was selected to be coated with low-melting point agarose, and the coated liver tissue block was placed in the specimen tank of a microtome containing L-15 culture medium for sectioning, and the thickness of the slice was 300μm, continuous oxygen. After slicing is completed, select the complete slice and place it in 1 mL of L-15 medium (containing 10% newborn calf serum, 2.5 μg / mL amphotericin, 0.1 mg / mL streptomycin, 100 IU / mL penicillin, pH7.0) In a 12-well plate, 6 pieces / well, put in 5% CO 2 , Shaking culture in a 27°C incubator.

[0018] The obtained liver slices were replaced with L-15 medium conta...

Embodiment 2

[0027] Screening experiment of fish hepatoprotective drugs

[0028] Step 1, using a vibrating microtome to prepare carp precision liver tissue slices, the slice thickness is 300 μm;

[0029] Step 2, the fine liver tissue slices obtained in step 1 are randomly divided into normal control group, TCDD model control group and test drug group, wherein the test drug group includes high, medium and low dose groups of licorice polysaccharide (200, 400 , 800 μg / mL) and glycyrrhizic acid high, medium and low dose groups (6.25, 12.5, 25 μg / mL), each group of the normal control group, TCDD model control group and the high, medium and low dose groups of the test drug was 6 slices of liver tissue;

[0030] Step 3, the normal control group and TCDD model group were treated with L-15 medium in 5% CO 2 , in a 27°C incubator with shaking culture, the test drug group was treated with high, medium and low doses (200, 400, 800 μg / mL) glycyrrhizin polysaccharide and high, medium and low doses (6....

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Abstract

The invention aims at providing a carp liver injury model which is used for screening liver-protecting medicines and an application thereof. The carp liver injury model is established by treatment on a carp liver tissue section by using 2,3,7,8-tetrachlorodibenzo-p-dioxin, the liver injury model is further treated by using a detection medicine and the treatment effect of the medicine on carp liver injury is judged. The carp liver injury model established according to the invention has the advantages of consistent sources, controllable culture environment, good repeatability, capability of screening various medicines in the same batch, short period and effect of greatly saving time and cost required for screening the medicines.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a carp liver injury model and the application of the model to screening liver-protecting drugs. Background technique [0002] 2,3,7,8-tetrachlorodiphenyl-p-dioxin (TCDD) is a highly toxic compound, mainly derived from industrial waste and pesticide abuse, etc., it not only brings huge It also poses a serious threat to ecological balance and human health. The study of the toxicity mechanism of dioxins has become one of the current scientific research hotspots, but there are still relatively few reports on the toxicity mechanism of dioxins. TCDD is a stable and persistent environmental pollutant that has negative effects on fish. Fish are particularly sensitive to water pollution, and the absorption of pollutants by fish tissues will affect many of its physiological and biochemical functions. Normally, TCDD is biotransformed in the liver, so the liver is the main storage site and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12Q1/02
Inventor 杜金梁殷国俊曹丽萍刘英娟王加豪
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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