Complete device for collagen purification

Abstract

The invention provides a complete device for collagen purification, which comprises a dissolution tank, a storage tank, a refining tank and a freezing dryer sequentially connected by pipelines, wherein a stirring blade is arranged in the dissolution tank; a pump and a centrifuge are arranged in the pipeline connecting the dissolution tank and the storage tank; a pump and a separation column are arranged in the pipeline connecting the storage tank and the refining tank; a film for filtering the crude extract is arranged on the separation column; the upper part of the separation column is provided with a pipeline for leading in a buffer solution; a pump is arranged on the pipeline for leading in the buffer solution; the refining tank is circularly connected with an ultrafiltration system through a pipeline; a pump is arranged in the pipeline connecting the refining tank and the ultrafiltration system; the refining tank is provided with a pipeline for leading in double distilled water, and a pump is arranged in the pipeline. The device provided by the invention improves the extraction rate of collagen, and foreign bacteria are not easily taken into the preparation process, thus the pollution is reduced, and the product quality is improved.

Description

technical field [0001] The invention relates to a complete set of equipment for collagen protein purification. Background technique [0002] Collagen is the most widely used biological material in the world, and the current international annual output is about 20,000 tons. In the medical field, collagen, as a highly biocompatible and highly bioactive material, is widely used in wound control and translational medicine. In 2011, the market research data of Frost & Sullivan, an internationally renowned business evaluation agency, also showed that collagen has become the most competitive biomedical material for regenerative medicine, and will further develop to become the two major materials of organ replacement medicine One, its technology readiness level - Technology readiness level (TRL) score is 8 (out of 9). The US Food and Drug Administration (FDA) predicts that collagen will occupy more than 20% of the biomedical materials market by 2015, with annual sales exceedin...

AI Technical Summary

Problems solved by technology

Although more than 90% of animal tendon is collagen, the process of stripping it from the animal body is labor-intensive and difficult to clean, resulting in final contamination of the product, especially the contamination of animal blood and muscle tissue that is difficult to remove

In addition, proteases in many animal tissues still exist. During the acid hydrolysis process, they will act on collagen at the same time as acid, resulting in irregular hydrolysis of collagen, which greatly reduces the controllability and repeatability of the process, and directly affects The quality of collagen, especially its special biological functionality

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