A kind of hemp bast fiber extract with antibacterial activity, preparation method and application thereof
A bast fiber and extract technology, which is applied in the comprehensive utilization technology of hemp and textile application field, can solve the problem of less research on active substances, and achieve the effect of excellent antibacterial performance.
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preparation example 1
[0052] Preparation Example 1: Silica gel adsorption column packing
[0053] Soak 200-300-mesh silica gel in chloroform and fully swell until it no longer swells, then pack the column and settle overnight to obtain a silica gel column.
[0054] Unless otherwise specified, the silica gel column used in all the following examples is the silica gel column packed in Preparation Example 1.
preparation example 2
[0055] Preparation Example 2: Silica gel adsorption column packing
[0056] 200-300 mesh silica gel was directly loaded into the column, and settled overnight to obtain a silica gel column, which was not fully swollen with chloroform.
Embodiment 1
[0058] (1) Washing the hemp bast fibers with water at room temperature to remove surface impurities and / or dirt, thereby obtaining clean hemp bast fibers.
[0059] (2) Vacuum-dry the clean hemp bast fiber at 40° C., and then shred it into small pieces with an area less than 1 cm×1 cm.
[0060] (3) Add ethanol that is 10 times the weight of the hemp bast fiber to the shredded hemp bast fiber, and stir and extract at 60°C for 4 hours. The extraction process can be carried out once or 2-4 times , after extraction, filter and collect the filtrate (when performing multiple times, each filtrate is combined).
[0061] (4) Use a rotary evaporator to concentrate the filtrate obtained in step (3) under reduced pressure, remove the solvent, and obtain the initial extract.
[0062] (5) Fully shake, dissolve, and suction filter the initial extract extract with a saturated NaCl aqueous solution to obtain an aqueous phase and undissolved solids; fully shake and dissolve the undissolved soli...
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