Method for producing ethanol by synchronous saccharification and fermentation of cellulose industrial waste residue
A technology of simultaneous saccharification and fermentation, industrial waste residues, applied in fermentation, biofuel and other directions, can solve the problems of low ethanol yield and difficult saccharification of cellulose
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Embodiment 1
[0039] The industrial waste residue derived from corn stalks is used as raw material, the cellulose content is 58%, and the moisture content of the wet residue is 70%. Add 7.33kg of deionized water to the industrial waste residue of 2.67kg of corn stalks to obtain a reaction system with a mass fraction of 10kg of waste residue with a mass fraction of 8%, and adjust the pH value of the system to 5.0; The reaction system with a volume of 5.0 is put into a fermenter with a volume of 15L, and cellulase liquid is added therein for fermentation. The cellulase is a cellulase with a preservation number of CCTCC M2011195. For cellulose, 2g of dry yeast, 5g of urea and 50g of corn steep liquor were added to the fermenter, and the fermentation was carried out at 38°C, and the fermentation was completed after 72 hours.
[0040] The present invention detects the concentration of ethanol after fermentation, and the result shows that the concentration of ethanol is 2.34%, and the calculated ...
Embodiment 2
[0042] The industrial waste residue derived from rice husk is used as raw material, the cellulose content is 60%, and the moisture content of wet residue is 78%. Add 5.46kg deionized water to the industrial waste residue of 4.54kg of this rice husk, obtain the reaction system that the massfraction that the mass fraction of 10kg waste residue absolute dry component is 10% is 10%, and the pH value of this reaction system is adjusted to 5.5; The reaction system with a pH value of 5.5 was put into a 15L fermenter, and commercially available cellulase was added therein. The amount of cellulase added was 10FPU / g cellulose, and 1g of dry yeast, 10g of urea and 75g of corn steep liquor was fermented at 40°C, and the fermentation ended after 72 hours;
[0043] The present invention detects the concentration of ethanol after fermentation, and the result shows that the concentration of ethanol is 2.94%, and the calculated yield of cellulose wine is 49%, which is 87.5% of the theoretical ...
Embodiment 3
[0045] The industrial waste residue derived from corn cobs is used as raw material, the cellulose content is 70%, and the moisture content of wet residue is 75%. Add 6kg of deionized water to the industrial waste residue of 4kg of this corncob to obtain a reaction system in which the mass fraction of 10kg of waste residue absolute dry component is 10%, and the pH value of the system is adjusted to 5.0; the obtained pH value is The reaction system of 5.0 is put into a fermenter with a volume of 15L, and commercially available cellulase is added thereto. The amount of cellulase added is 15FPU / g cellulose, and 1g dry yeast, 7g urea and 50g corn steep liquor are also added thereto. Fermentation was carried out at 42°C, and the fermentation ended after 72 hours.
[0046] The present invention detects the concentration of ethanol obtained after fermentation, and the result shows that the concentration of ethanol is 3.22%, and the calculated yield of cellulose wine is 46%, which is 8...
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Abstract
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