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Preparation method of chitosan by fermentation

A chitosan and drying technology, applied in the field of manufacturing, can solve the problems of high concentration of lye, waste, and serious environmental pollution, and achieve the effects of low energy consumption, avoiding waste of resources, and reducing the amount of strong acid

Active Publication Date: 2016-05-11
TIANJIN TIANSHI BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high concentration of lye used in the chemical method, the reaction time is long, the product quality is unstable, and the environmental pollution is serious
Chitosan prepared by traditional chemical methods after decalcification and deproteinization is discarded and cannot be recycled, resulting in waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dry and crush crab or shrimp shells to 0.5mm particles, add 4 times the weight-to-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria accounted for The weight ratio of raw materials is 0.02%, fermented for 24 hours, then 70°C for 20 minutes; the fermentation broth is filtered, the residue is collected, and the filtrate is recycled and dried to produce bio-organic calcium. Add 8% hydrochloric acid to the residue for decalcification, after fully reacting, filter, wash with water until neutral, and keep the residue. Add 8% sodium hydroxide solution to the residue, 80°C, 1h, filter and wash with water until neutral. Add 40% sodium hydroxide solution to the residue, wash at 60°C for 1.5h, until neutral, and dry.

Embodiment 2

[0022] Dry and crush crab or shrimp shells to 1.0mm particles, add 8 times the weight-to-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria account for The weight ratio of raw materials is 0.06%, fermented for 36 hours, and then 100°C for 20 minutes; the fermentation broth is filtered, the residue is collected, and the filtrate is recovered and dried to produce bio-organic calcium. Add 15% hydrochloric acid to the residue for decalcification, after fully reacting, filter, wash with water until neutral, and keep the residue. Add 15% sodium hydroxide solution to the residue, 100°C, 3h, filter and wash with water until neutral. Add 60% sodium hydroxide solution to the residue, wash at 90°C for 4 hours until neutral, and dry.

Embodiment 3

[0024] Dry and crush the crab or shrimp shells to 0.5mm particles, add 8 times the weight-volume ratio of water to make a feed liquid, add Bifidobacterium lactis: Lactobacillus bulgaricus: Lactobacillus helveticus = 2:6:5 lactic acid bacteria, lactic acid bacteria accounted for The weight ratio of raw materials is 0.04%, fermented for 36 hours, then 80°C for 20 minutes; the fermentation broth is filtered, the residue is collected, and the filtrate is recycled and dried to produce bio-organic calcium. Add 10% hydrochloric acid to the residue for decalcification, after fully reacting, filter, wash with water until neutral, and keep the residue. Add 10% sodium hydroxide solution to the residue, 90°C, 3h, filter and wash with water until neutral. Add 50% sodium hydroxide solution to the residue, wash at 80°C for 3 hours until neutral, and dry.

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Abstract

The invention discloses a method for preparing chitosan by fermentation, which comprises the following steps: drying and pulverizing crab shells or shrimp shells; fermenting; decalcifying; deproteinizing; deacetylating, washing with water, drying and pulverizing. Compared with the prior art, the enzymolysis process of the present invention adopts two decalcifications, reduces the amount of strong acid, has low energy consumption, no pollution, and is more environmentally friendly. After the fermentation broth is recycled, it can be made into biological osteocalcin, so that all products can be rationally utilized to generate significant economic value and avoid waste of resources.

Description

technical field [0001] The invention relates to a preparation method of chitosan and belongs to the field of manufacturing. Background technique [0002] Chitin is a natural nitrogen-containing polysaccharide substance, which widely exists in the shell or cuticle of invertebrates, especially in the shells of shrimp and crabs. Chitin is easy to obtain and renewable. It is a natural homopolymer second only to cellulose. However, due to the strong hydrogen bond in the molecule, the solubility is low and the application range is limited. [0003] Chitosan is formed after deacetylation of chitin. At present, chitosan is usually prepared by chemical and enzymatic deacetylation. In the chemical deacetylation reaction, there are many factors affecting the deacetylation, but the main influencing factors include: lye concentration, reaction temperature and time. In general, increasing the reaction temperature and prolonging the reaction time can increase the degree of deacetylation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 黄永亮李莹崔卜东郁正刚田志同杨国元
Owner TIANJIN TIANSHI BIOLOGICAL DEV
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