A kind of method for transplanting virus-free tissue-cultured seedlings of Lycium barbarum

Abstract

The invention provides a medlar virus-free tissue culture seedling transplanting method, which comprises the following steps that the illumination period of 2-3-weak-old tissue culture seedlings is regulated to a state with 8-hour illumination and 16-hour darkness; meanwhile, the illumination intensity during the illumination is lowered to 100 to 500lux; after the tissue culture seedlings are treated for 7 to 10 days, the tissue culture seedlings in a tissue culture bottle are taken out, the tissue culture seedlings are sheared down along the surface of a culture medium, base part blades are moved, two to three leaves at the top are remained, parts being 0 to 2cm from the lower ends of the stems of the tissue culture seedlings are fast dipped with a rooting solution, water is uniformly sprayed on the leaf surfaces, and the wetness of the stems and the leaves is maintained; cell trays are taken, seedling culture nutrient soil is filled in the cell trays, sufficient water solution is watered, holes are formed, and the tissue culture seedlings are planted; then, support frames are arranged on the cell trays, and plastic films are mulched; the cell trays after the transplanting are placed into a screen greenhouse, wherein the screen greenhouse is respectively covered by nylon screens being more than 40 meshes in spring, summer and autumn; after the tissue culture seedlings are transplanted for 7 to 10 days, the seedlings are put in the wind for exercising the seedlings, and leaf fertilizers are sprayed; after the seedlings are transplanted for 30 to 60 days, the tissue culture seedlings grow healthy and strongly, and the provisonal planting can be carried out.

Description

technical field [0001] The invention relates to the technical field of protected cultivation, in particular to a method for transplanting virus-free tissue-cultured seedlings of Lycium barbarum. Background technique [0002] Lycium chinense (Lycium chinense) is a multi-branched shrub of the genus Lycium in the Solanaceae family. It is rich in various nutrients such as Lycium proteoglycan, VC, phosphorus, and iron. value. At present, the breeding technology of wolfberry is mostly hardwood cuttings and softwood cuttings in production. Hardwood cuttings and softwood cuttings retain the excellent traits of the mother, and also retain the plant viruses in the mother. The accumulation of viruses in wolfberry increases from generation to generation. It continues to affect the per unit yield of wolfberry, and the quality of wolfberry fruit is declining year by year. [0003] Prior art Lycium barbarum virus-free tissue culture rapid propagation is divided into five stages: (1) obta...

AI Technical Summary

Problems solved by technology

[0004] 1. Tissue culture seedlings grow indoors for a long time, and consume high energy and space, resulting in high production costs

[0005] 2. The cleaning process of tissue culture seedling medium consumes more water resources, and the root system is easily damaged during the cleaning process; after hardening, the tissue culture seedlings have a high degree of lignification, and it is difficult to regenerate the root system, resulting in a low survival rate

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