Method for removing bitterness of linseed oil by enzymatic process
A linseed oil removal technology, applied in the direction of edible oil/fat, food science, application, etc., can solve the problems of lack of market competitiveness of products, high cost of bitterness removal, etc., and achieve easy promotion, low environmental requirements, and simple operation Effect
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[0020] Example 1
[0021] First take 1000ml of bitter linseed oil and heat it to 50℃; then take 1g of neutral protease with enzyme activity of 200,000 u / g and dissolve it in 8ml of drinking water at 50℃, adjust the pH of the solution to 7; then prepare Add the neutral protease solution to 1000ml of hot oil while stirring, continue stirring for 3 hours, then heat the oil to 85°C to inactivate the enzyme for 30 minutes, centrifuge the oil and water, and finally dehydrate the oil obtained by centrifugation to obtain Linseed oil without the bitter taste. After calculation, the cost of reagents for debittering treatment is 0.1 yuan / kg of linseed oil.
Example Embodiment
[0022] Example 2
[0023] Take 1000ml of freshly squeezed linseed oil and heat it to 55°C; then take 0.1g of neutral protease with an enzyme activity of 200,000 u / g and dissolve it in 2ml of drinking water at 55°C, and adjust the pH of the solution to 7.2; Add the prepared neutral protease solution to 1000ml of hot oil while stirring, continue stirring for 4 hours, then heat the oil to 80°C to inactivate the enzyme for 30 minutes, centrifuge the oil and water, and finally dehydrate the oil obtained by centrifugation. The linseed oil with the bitter taste removed is obtained.
Example Embodiment
[0024] Example 3
[0025] Take 1000ml of bitter linseed oil and heat it to 40°C; then take 2g of neutral protease with enzyme activity of 200,000 u / g and dissolve it in 20ml of drinking water at 48°C, adjust the pH of the solution to 7.2; Add the neutral protease solution to 1000ml of hot oil during stirring, continue stirring for 2 hours, then heat the oil to 82°C to inactivate the enzyme for 30 minutes, centrifuge to separate the oil and water, and finally dehydrate the oil obtained by centrifugation. Linseed oil to remove bitterness.
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