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Extraction and separation method of β-carotene in daffodils

A carotene and separation method technology, which is applied in the field of pigment extraction in plants, can solve the problems that the extraction and separation methods of β-carotene have not been reported yet, and achieve the effect of simple operation, wide sources and high purity

Inactive Publication Date: 2016-08-24
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction and separation methods for high-purity β-carotene in carotenoids have not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The kind of daffodils involved in this embodiment is Narcissus pseudonarcissus 'Prof. Einstein'.

[0031] The present embodiment relates to a method for extracting and separating β-carotene in daffodils, comprising the following steps:

[0032] Step 1, take fresh paracrown tissue of daffodils, add liquid nitrogen to freeze-dry and grind to powder, add pigment extraction solution, ultrasonic extraction;

[0033] Step 2, add n-hexane with a solid-liquid ratio (g:ml) of 1:5, extract until the lower layer is colorless, and combine the extracts;

[0034] Step 3, adding a saturated NaCl solution with a solid-to-liquid ratio (g:ml) of 1:10 into the extract for washing, making the supernatant neutral, and discarding the lower layer;

[0035] Step 4, placing the extract in a vacuum drying oven to concentrate in vacuo until the solvent is evaporated;

[0036] Step 5, adding a methyl tert-butyl ether solution with a solid-liquid ratio (g:ml) of 1:2 to dissolve, and simultaneousl...

Embodiment 2

[0042] The kind of daffodils involved in this embodiment is Narcissus pseudonarcissus 'Prof. Einstein'.

[0043] The present embodiment relates to a method for extracting and separating β-carotene in daffodils, comprising the following steps:

[0044] Step 1, take fresh paracrown tissue of daffodils, add liquid nitrogen to freeze-dry and grind to powder, add pigment extraction solution, ultrasonic extraction;

[0045] Step 2, add n-hexane with a solid-liquid ratio (g:ml) of 1.5:10, extract until the lower layer is colorless, and combine the extracts;

[0046] Step 3, adding a saturated NaCl solution with a solid-to-liquid ratio (g:ml) of 1.5:20 into the extract for washing, making the supernatant neutral, and discarding the lower layer;

[0047] Step 4, placing the extract in a vacuum drying oven to concentrate in vacuo until the solvent is evaporated;

[0048] Step 5, adding a methyl tert-butyl ether solution with a solid-liquid ratio (g:ml) of 1.5:3 for dissolving, and add...

Embodiment 3

[0054]The kind of daffodils involved in this embodiment is Narcissus pseudonarcissus 'Prof. Einstein'.

[0055] The present embodiment relates to a method for extracting and separating β-carotene in daffodils, comprising the following steps:

[0056] Step 1, take fresh paracrown tissue of daffodils, add liquid nitrogen to freeze-dry and grind to powder, add pigment extraction solution, ultrasonic extraction;

[0057] Step 2, add n-hexane with a solid-liquid ratio (g:ml) of 1:10, extract until the lower layer is colorless, and combine the extracts;

[0058] Step 3, adding a saturated NaCl solution with a solid-to-liquid ratio (g:ml) of 1:15 into the extract for washing, making the supernatant neutral, and discarding the lower layer;

[0059] Step 4, placing the extract in a vacuum drying oven to concentrate in vacuo until the solvent is evaporated;

[0060] Step 5, adding a methyl tert-butyl ether solution with a solid-liquid ratio (g:ml) of 1:2 to dissolve, and adding a 10% ...

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Abstract

The invention provides a method for extracting and separating β-carotene in daffodils; it includes the following steps: step 1, taking the paracrown tissue of daffodils, freeze-drying and grinding to powder, adding pigment extract, and ultrasonically extracting; step 2. Add n-hexane for extraction, combine and wash the extracts, centrifuge, concentrate in vacuum, saponify, wash twice, concentrate twice, and dissolve. The purity of the β-carotene sample liquid obtained by the method of the invention reaches more than 98%, and can be directly used as a high-performance liquid chromatography grade standard product. The invention has wide sources of materials, simple and convenient operation, low cost and high purity, and is suitable for the preparation of Tianyuan β-carotene standard products.

Description

technical field [0001] The invention relates to a method for extracting pigments in plants, in particular to a method for extracting and separating β-carotene from daffodils. Background technique [0002] Narcissus is a perennial herb of the genus Narcissus in the family Amaryllidacea, and is an important ornamental flower. Studies have found that the most important pigment components in daffodils are carotenoids. Carotenoids are an important class of natural pigments, mainly including carotene and lutein. Among them, β-carotene is the most important compound of carotene, and has a wide range of anti-oxidation, anti-tumor and anti-inflammatory effects. [0003] β-carotene is widely used as a standard in the qualitative and quantitative analysis of carotenoids, but it is expensive due to its complex extraction process, difficult synthesis, high cost, and easy oxidation and photolysis. At present, some related reports on obtaining carotenoids from plants have been found, su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C403/24
Inventor 李欣史益敏卢敏
Owner SHANGHAI JIAOTONG UNIV