Hericium erinaceus cell wall polysaccharide and preparation method thereof

A technology of Hericium erinaceus and cell wall is applied in the field of Hericium erinaceus cell wall polysaccharide and its preparation, which can solve the problems such as the lack of systematic and comprehensive reports on the extraction of cell wall polysaccharide, and achieve the effects of high yield, improved utilization rate and light color.

Active Publication Date: 2014-09-24
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research and utilization of Hericium erinaceus polysaccharides are mainly aimed at water extraction of intracellular polysaccharides. Ther

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1:

[0024] Extraction of water-soluble Hericium erinaceus cell wall polysaccharides:

[0025] Hericium erinaceus fruiting bodies were purchased from Shanghai Guosen Biological Technology Co., Ltd.

[0026] Add Hericium erinaceus to 15 times the weight of water, soak for 30 minutes at room temperature, heat to boiling, keep for 120 minutes, filter, collect the residue, repeat the above steps until the intracellular polysaccharide is extracted completely, and collect residue 1;

[0027] Crush residue 1 for 15 minutes (200-300 mesh size), weigh 200g, add 4L of water, soak at room temperature for 30min, heat to boiling, hold for 120min, centrifuge at 9803g for 15min, precipitate and repeat the above steps, extract 2 more times, combine the extracts , Save the residue 2 for future use;

[0028] The extract was centrifuged at high speed and concentrated to a ratio of material to liquid of 1:5, and then added with absolute ethanol until the final concentration of ethanol reach...

Example Embodiment

[0039] Example 2:

[0040] Determination of the activity of cell wall polysaccharides to stimulate macrophages to release NO in vitro

[0041] 1. Sample preparation: Weigh the Hericium erinaceus cell wall polysaccharide prepared in Example 1 into a sterilized eppendorf tube, and prepare a solution with a concentration of 5 mg / mL with sterile PBS. After being fully dissolved, it was centrifuged at 15000r / min for 30min, transferred to a new sterile eppendorf tube under aseptic conditions, and the sample was diluted to 2mg / ml and 0.5mg / mL for later use.

[0042] 2. Cell culture: Take the logarithmic growth phase RAW264.7 macrophage cell line (purchased from the Institute of Cells, Chinese Academy of Sciences), and subculture in DMEM complete medium (purchased from Gibco) at 37°C and 5% CO2. Digest with 0.05% trypsin or 5% EDTA solution, centrifuge the suspension at 1000 rpm / min for 3 minutes, collect the cells, and count them for later use.

[0043] 3. Reagent preparation and standard c...

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PUM

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Abstract

The invention provides hericium erinaceus cell wall polysaccharide and a preparation method thereof. The hericium erinaceus cell wall polysaccharide comprises water soluble cell wall polysaccharide and alkali soluble cell wall polysaccharide, and is prepared through removing the polysaccharide in the cell, smashing and water extracting, alkali extracting and alcohol precipitating. The method fully extracts the cell wall polysaccharide in the hericium erinaceus, can obtain high yield of high content cell wall polysaccharide, can obviously promote RAW264.7 macrophage cell line to release NO, and has in vitro immunological competence.

Description

technical field [0001] The invention relates to the field of edible and medicinal fungus extraction, in particular to a kind of Hericium erinaceus cell wall polysaccharide and its preparation method Background technique [0002] Hericium erinaceus (Hericium erinaceus), belongs to the kingdom of fungi in taxonomy, Basidiomycota, Basidiomycetes, Hericium erinaceae, Hericium erinaceae, Hericium erinaceus, also known as Hericium erinaceus, bear's head mushroom, hedgehog , tender and delicious meat, rich in nutrition, is a well-known medicinal and edible fungus, and has been widely used in China to treat digestive system diseases such as indigestion, gastric ulcer, esophageal cancer, gastric cancer, duodenal cancer, and cardia cancer. Hericium erinaceus polysaccharide is the main active ingredient of Hericium erinaceus. Studies have shown that Hericium erinaceus polysaccharide has the effects of anti-tumor, improving immunity, lowering blood sugar, anti-mutation, and anti-aging. ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 杨焱唐川吴迪刘艳芳张劲松颜梦秋唐传红唐庆九周帅张忠
Owner SHANGHAI ACAD OF AGRI SCI
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