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Hericium erinaceus cell wall polysaccharide and preparation method thereof

A technology of Hericium erinaceus and cell wall is applied in the field of Hericium erinaceus cell wall polysaccharide and its preparation, which can solve the problems such as the lack of systematic and comprehensive reports on the extraction of cell wall polysaccharide, and achieve the effects of high yield, improved utilization rate and light color.

Active Publication Date: 2014-09-24
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research and utilization of Hericium erinaceus polysaccharides are mainly aimed at water extraction of intracellular polysaccharides. There have been many reports on the related preparation processes, but there is no systematic and comprehensive report on the extraction of cell wall polysaccharides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Extraction of water-soluble Hericium erinaceus cell wall polysaccharide:

[0025] The fruiting bodies of Hericium erinaceus were purchased from Shanghai Guosen Biotechnology Co., Ltd.

[0026] Add Hericium erinaceus to 15 times the weight of water, soak at room temperature for 30 minutes, heat to boiling, keep for 120 minutes, filter, collect the residue, repeat the above steps until the intracellular polysaccharide is completely extracted, and collect the residue 1;

[0027] Crush residue 1 for 15 minutes (crushing particle size 200-300 mesh), weigh 200 g, add 4 L of water, soak at room temperature for 30 minutes, heat to boiling, keep for 120 minutes, centrifuge at 9803 g for 15 minutes, repeat the above steps for precipitation, and then extract twice, and combine the extracts , keep the residue 2 for subsequent use;

[0028] The extract was centrifuged at high speed, concentrated to a solid-liquid ratio of 1:5, and then added into absolute ethanol until the final co...

Embodiment 2

[0040] Determination of the Activity of Cell Wall Polysaccharides Stimulating Macrophages to Release NO in Vitro

[0041] 1. Sample preparation: Weigh the cell wall polysaccharide of Hericium erinaceus prepared in Example 1 into a sterilized eppendorf tube, and prepare a solution with a concentration of 5 mg / mL with sterile PBS. After fully dissolving, centrifuge at 15000r / min for 30min, transfer to a new sterile eppendorf tube under aseptic conditions, and dilute the sample to 2mg / ml, 0.5mg / mL for use.

[0042] 2. Cell culture: Take the RAW264.7 macrophage cell line in the logarithmic growth phase (purchased from the Institute of Cells, Chinese Academy of Sciences), and use DMEM complete medium (purchased from Gibco) to subculture at 37 ° C and 5% CO2. Digest with 0.05% trypsin or 5% EDTA solution, collect the cells after the suspension is centrifuged at 1000rpm / min for 3min, and count for future use.

[0043] 3. Reagent preparation and standard curve drawing

[0044] The p...

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Abstract

The invention provides hericium erinaceus cell wall polysaccharide and a preparation method thereof. The hericium erinaceus cell wall polysaccharide comprises water soluble cell wall polysaccharide and alkali soluble cell wall polysaccharide, and is prepared through removing the polysaccharide in the cell, smashing and water extracting, alkali extracting and alcohol precipitating. The method fully extracts the cell wall polysaccharide in the hericium erinaceus, can obtain high yield of high content cell wall polysaccharide, can obviously promote RAW264.7 macrophage cell line to release NO, and has in vitro immunological competence.

Description

technical field [0001] The invention relates to the field of edible and medicinal fungus extraction, in particular to a kind of Hericium erinaceus cell wall polysaccharide and its preparation method Background technique [0002] Hericium erinaceus (Hericium erinaceus), belongs to the kingdom of fungi in taxonomy, Basidiomycota, Basidiomycetes, Hericium erinaceae, Hericium erinaceae, Hericium erinaceus, also known as Hericium erinaceus, bear's head mushroom, hedgehog , tender and delicious meat, rich in nutrition, is a well-known medicinal and edible fungus, and has been widely used in China to treat digestive system diseases such as indigestion, gastric ulcer, esophageal cancer, gastric cancer, duodenal cancer, and cardia cancer. Hericium erinaceus polysaccharide is the main active ingredient of Hericium erinaceus. Studies have shown that Hericium erinaceus polysaccharide has the effects of anti-tumor, improving immunity, lowering blood sugar, anti-mutation, and anti-aging. ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 杨焱唐川吴迪刘艳芳张劲松颜梦秋唐传红唐庆九周帅张忠
Owner SHANGHAI ACAD OF AGRI SCI
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