Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of fxyd in the preparation of markers for diagnosis and prediction of lung cancer and fxyd double antibody sandwich ELISA detection kit

A kit and reagent technology, applied in the field of detection, to achieve good specificity, convenient clinical diagnosis and prediction of lung cancer, and high sensitivity

Active Publication Date: 2016-02-10
SHANGHAI LIANGRUN BIOMEDICINE TECH CO LTD
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the correlation between FXYD and lung cancer has not been reported so far

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of fxyd in the preparation of markers for diagnosis and prediction of lung cancer and fxyd double antibody sandwich ELISA detection kit
  • Application of fxyd in the preparation of markers for diagnosis and prediction of lung cancer and fxyd double antibody sandwich ELISA detection kit
  • Application of fxyd in the preparation of markers for diagnosis and prediction of lung cancer and fxyd double antibody sandwich ELISA detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Establishment of FXYD's double-antibody sandwich ELISA detection system

[0022] Anti-FDP polyclonal antibody (purchased from LifeSpan Biosciences), anti-FDP monoclonal antibody (purchased from ADI), anti-fibrinogen (Fibrinogen, FIB) polyclonal antibody (purchased from LifeSpan Biosciences) with a concentration of 1 μg / mL Coat the ELISA plate with coating solution, coat overnight at 4°C, wash the plate; then block in BSA with a mass fraction of 2% at 37°C for 2 hours, spin dry at 37°C for half an hour; then apply the blocked enzyme label Add 0.5 μg / mL FDP standard (purchased from Shanghai Sun Biotechnology Co., Ltd.) to the plate, react at 37°C for 1 hour, and wash the plate; add 0.1 μg / mL of HRP-labeled Fibrinogenγ antibody (B-1) and Fibrinogenα antibody (A-6) (Fibrinogen γ antibody (B-1) and Fibrinogen α antibody (A-6) were purchased from Santa Cruz Biotechnology Company) mixture was added to the microtiter plate, 37 ° C for 1 hour, washed plate; added colo...

Embodiment 2

[0028] According to the FXYD double-antibody sandwich ELISA detection system established in Example 1, the FXYD double-antibody sandwich ELISA detection kit was constructed, as shown in Table 1:

[0029] Table 1. FXYD Double Antibody Sandwich ELISA Kit

[0030]

[0031] The constructed FXYD double-antibody sandwich ELISA detection kit was used to detect the concentration of 0, 12.5ng / mL, 25ng / mL, 50ng / mL, 100ng / mL, 200ng / mL, 400ng / mL FDP standard and volume ratio of Serum samples diluted 1:100 were tested for OD readings at 450nm after the reaction, and the results were as follows figure 1 shown. Depend on figure 1 It can be seen that the linear range of the constructed FXYD double-antibody sandwich ELISA detection kit is 12.5ng / mL~400ng / mL, the linear correlation coefficient in the linear range is R>0.990, the detection recovery rate is in the range of 90%~110%, and the lower detection limit 7.5ng / mL.

[0032]Evaluate the FXYD double-antibody sandwich ELISA detection k...

Embodiment 3

[0033] Example 3, FXYD Double Antibody Sandwich ELISA Detection Kit for Diagnosis and Prediction of Lung Cancer

[0034] 1. FXYD Double Antibody Sandwich ELISA Kit for Diagnosis of Lung Cancer

[0035] Serum from 150 cases of preoperative lung cancer was collected from Shanghai Cancer Hospital, and 300 cases of healthy blood donors were collected from the blood bank, with 1 mL of serum per case. Use the FXYD double-antibody sandwich ELISA detection kit to detect the concentration of FXYD in the serum of lung cancer patients and healthy people, and draw the receiver operating characteristic curve (ROC curve) according to the detection results ( figure 2 ). Then the area under the curve, sensitivity and specificity of double-antibody sandwich detection of lung cancer marker FXYD were counted according to ROC curve. The results showed that the cutoff value of the FXYD double-antibody sandwich ELISA detection kit for detecting FXYD markers to distinguish lung cancer from normal...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention discloses application of a fibrinogen degradation product FXYD in preparation of a marker for diagnosing and indicating lung cancer and an FXYD double-antibody sandwich ELISA (enzyme-linked immune sorbent assay) detection kit, wherein FXYD is a mixture with amino acid sequences shown in SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4; the kit comprises a solid phase carrier coated with a trapping agent for trapping the fibrinogen degradation product FXYD, an enzyme-labeling anti-FXYD polyclonal antibody, a developing substrate and stopping liquor, can be used for monitoring lung cancer diagnosis, therapeutic effect evaluation and metastasis recurrence, so that blank in FXYD lung cancer auxiliary diagnosis is filled up.

Description

technical field [0001] The invention belongs to the detection field, and specifically relates to the application of fibrinogen degradation product FXYD in preparing markers for diagnosis and prediction of lung cancer, and also relates to a double-antibody sandwich ELISA detection kit for detecting fibrinogen degradation product FXYD. Background technique [0002] At present, lung cancer is one of the malignant tumors with the fastest-growing morbidity and mortality and the greatest threat to the health and life of the population. In the past 50 years, many countries have reported that the morbidity and mortality of lung cancer have increased significantly. With the development of the economy, our country The deteriorating environmental quality, especially the continuous decline of air quality may be one of the reasons leading to the rising incidence of lung cancer. Clinical studies have found that more than 90% of cancer cases in China are discovered after the second stage. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/57423G01N33/6803G01N2333/75
Inventor 王弢周小进孙玉龙
Owner SHANGHAI LIANGRUN BIOMEDICINE TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com