Preparation method of lupeol

A technology for lupin alcohol and purpose is applied in the field of preparation of lupin alcohol, which can solve the problems of difficulty in large-scale extraction and separation, difficulty in preparation, separation and purification, and influence on research, test and promotion work.

Inactive Publication Date: 2014-10-29
SHAANXI LONGFU BIOLOGICAL CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preparative chromatography can reach a content of more than 99%, but it is difficult to meet the needs of large-scale production. Currently, it is only used for the separation of reference substances
Due to the difficulty of extraction and separation in large quantities
Affected further research and experiment promotion work
The difficulty of preparation, separation and purification leads to high purification cost, which greatly limits the extensive research and application of this product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 500g of Chinese wolfberry leaves with a content of 1.2%, crush them to 20 meshes, soak them in 3000ml of water at room temperature for 2 hours, remove the water soaking solution, and use 2500ml, 2000ml, 1800ml of 85% ethanol solution to repeatedly reflux and extract the water soaking residue three times, combine The extract was filtered and concentrated under reduced pressure at 70°C. Obtain 82 grams of medicinal extract, 82 grams of medicinal extract are extracted with 300ml n-butanol at normal temperature, and the extract obtained last time reclaims n-butanol. The extract was dried to obtain 16.5 g of dry product, and the content of lupeol was detected to be 34%. The dry product was stirred and dissolved with 30% ethanol solution to obtain 100 g of extract. Load the treated Diaon HP-20 resin 200g in the lower layer of the glass column, then slowly pour the dissolved extract solution into the column, rinse with 300% ethanol 300ml, then elute with 70% ethanol solu...

Embodiment 2

[0018]Soak, extract, and resin column the pulverized Chinese wolfberry leaves with a lupeol content of 68.5%. Dissolve 100 grams of the crude product in 600 ml of 80% ethanol, add concentrated hydrochloric acid until the final concentration of hydrochloric acid is 5%, stir in a water bath for 5 hours, and then cool to room temperature. , filter out the crystals, use 10% sodium hydroxide to neutralize the filtrate, recover the alcohol solution, and combine the crystals after drying and elute with chloroform-methanol to separate the crystals, detect the eluted components by thin layer, collect the target substance, concentrate and dry Then crystallize with absolute ethanol to obtain 75.2 grams of crystals with a content of 98.83%.

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PUM

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Abstract

The invention relates to a preparation method of lupeol. An acidizing technology is utilized to decompose and convert the saponin and acid ester derivative in part of the lupeol into lupeol, and destroy the impurities, so as to achieve the purpose of separation and preparation of lupeol. The macroporous resin bed is combined with a silica gel column chromatography to reduce the separation difficulty of lupeol, and increase the purification content of lupeol in holly leaf extract from the traditional 90% to 98%. At the same time, the method improves the utilization rate of resources, so that the content of lupeol achieves the demands of the markets home and abroad, and the scale production cost of lupeol is reduced.

Description

[0001] Technical field [0002] A preparation method involving a kind of lUPEOL (Lupeol), using acidified technology to make some saponin and acid ester derivatives of some feathers be transformed into fan beanol, and destroy the impurities part to achieve separation of fan beanol.Purpose.Combined with large -hole resin beds combined with silicone column layer techniques, it reduces the separation difficulty of the fan beanol. It can increase the purification content of the fan beanol in the extract of the wolf bone leaf from 90%to 98%.At the same time, the resource utilization rate has been improved, and the content of the fan of the feathers can meet the requirements of the domestic and foreign markets, and reduce the large -scale production cost of feathers. [0003] Background technique [0004] Lupol (LUPEOL) LUP-20 (29) -EN-3β-OL; 3β-Hydroxy -20 (29) – LUPENE, also known as snake moxol, snake moxol, snake alcohol ester.Broadly distributed in Douke, Yao Jinniang, Sang family...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 代维正
Owner SHAANXI LONGFU BIOLOGICAL CHEM CO LTD
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