Natural composite amino acid raw material preparation method and natural composite amino acid raw material
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A compound amino acid and raw material technology is applied in the field of preparation of natural compound amino acid raw materials, and achieves the effects of large sample loading, low cost, and simple and reliable method.
Inactive Publication Date: 2014-11-05
HUNAN LINUO BIOLOGICAL PHARMA
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Problems solved by technology
Those skilled in the art are eager to obtain a safe compound amino acid product, but this technical problem has not been solved
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Embodiment 1
[0030] The preparation method of this natural compound amino acid raw material of the present invention comprises the steps:
[0031]a. Take 50kg of frozen fresh pig brain, soak it in water to thaw naturally, then wash it with water, then add 100kg of purified water and use a colloid mill to homogenize it for 15 minutes, pour the slurry obtained from the homogenization into an extraction tank, heat it to 80°C, keep it warm for 15 minutes, and then Add 250 ml of concentrated hydrochloric acid with a concentration of 12 mol / L, stir evenly, cool to 40°C, centrifuge at a speed of 3800r / min, remove the upper emulsion, collect the precipitate, and obtain 2.3kg of precipitated cerebroprotein;
[0032] b. Add 3000ml of sulfuric acid with a concentration of 18.4mol / L to the precipitated protein obtained in step a for hydrolysis to make a sulfuric acid solution with a concentration of 12mol / L, heat to 121°C, hydrolyze for 8 hours, and then dilute with water 10 times the weight ratio , u...
Embodiment 2
[0038] a. Take 50kg of frozen fresh pig brain, soak it in water to thaw naturally, then wash it with water, then add 200kg of purified water and use a colloid mill to homogenize it for 15 minutes, pour the slurry obtained from the homogenization into an extraction tank, heat it to 70°C and keep it warm for 10 minutes, then Add 100 ml of concentrated hydrochloric acid with a concentration of 12mol / L, stir well, cool to 10°C, and then centrifuge at a speed of 3000r / min, then remove the upper emulsion, collect the precipitate, and obtain 2.4kg of precipitated cerebroprotein;
[0039] b. Add 2500ml of sulfuric acid with a concentration of 18.4mol / L to the precipitated protein obtained in step a for hydrolysis to make a sulfuric acid solution with a concentration of 6mol / L, heat to 110°C, hydrolyze for 6 hours, and then dilute with water with a weight ratio of 5 times , use calcium hydroxide solution to adjust the pH value to 8 (calcium hydroxide solution used is obtained by dissolv...
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Abstract
The invention discloses a natural composite amino acid raw material preparation method. The method comprises the following steps: 1, taking cryopreserved fresh pig brain, unfreezing, cleaning, homogenating, adding the above obtained slurry to concentrated hydrochloric acid, uniformly stirring, and centrifuging to obtain precipitated brain proteins; 2, adding the precipitated proteins to sulfuric acid, hydrolyzing, adjusting the pH value to 7.5-8.5, and filtering to obtain an acid hydrolysate; 3, heating the acid hydrolysate, adding active carbon, separating, collecting the above obtained supernatant, and adjusting the pH value to 2.5-4.5 to obtain a crude extract liquid; 4, adding the crude extract liquid to a cation exchange column, adjusting the pH value to 6.5-7.5, eluting with ammonia water, collecting the obtained eluate, concentrating to remove ammonia water, adjusting the pH value to 6.5-7.5, and preserving at -20DEG C to obtain a frozen liquid; and 5, naturally unfreezing the frozen liquid, centrifuging, removing tyrosine and related impurities, collecting the newly obtained supernatant, carrying out ultrafiltration on the supernatant, collecting the obtained filtrate, and drying to obtain the desired natural composite amino acid raw material.
Description
technical field [0001] The invention relates to a preparation method of a natural compound amino acid raw material, and also relates to a natural compound amino acid raw material. Background technique [0002] Amino acid (Amino acid) is the basic unit of protein, which endows the protein with a specific molecular structure and makes its molecules biochemically active. Proteins are important active molecules in organisms, including enzymes and enzymes that catalyze metabolism. Amino acid is the most basic substance that constitutes biological proteins and is related to life activities. It is the basic unit of protein molecules in organisms and has a close relationship with biological life activities. It has special physiological functions in antibodies and is one of the indispensable nutrients in living organisms. [0003] Amino acids can be roughly divided into three categories: [0004] 1. Essential amino acids: Refers to the human body or other vertebrates that cannot b...
Claims
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