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Buffer system suitable for continuous flow method for measuring total alkaloid in tobacco and tobacco products

A technology for tobacco products and plant alkaloids, which can be used in the measurement of color/spectral characteristics and analysis by chemical reactions of materials, which can solve the problems of large amount of chemicals and inconvenient use.

Active Publication Date: 2014-11-05
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In YC / T468-2013, there are also requirements for a suitable acid-base environment for the reaction. The acid-base environment of the reaction is also controlled by buffer solution A and buffer solution B. Although the acid-base environment of the reaction can be effectively controlled, the method buffer solution The amount of chemicals used in the preparation of A and buffer solution B is relatively large, which also brings inconvenience to the use of this method

Method used

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  • Buffer system suitable for continuous flow method for measuring total alkaloid in tobacco and tobacco products
  • Buffer system suitable for continuous flow method for measuring total alkaloid in tobacco and tobacco products
  • Buffer system suitable for continuous flow method for measuring total alkaloid in tobacco and tobacco products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 : Determination of total plant alkaloids in flue-cured tobacco

[0063] 1) Sample pretreatment

[0064] Prepare the flue-cured tobacco powder into a sample according to YC / T31, and measure its moisture content, then weigh 0.25g of the sample in a 50mL Erlenmeyer flask with a stopper, add 25mL of 5% acetic acid, cover the stopper, and vibrate on a shaker (rotating speed >150rpm) extraction for 30min. Filter the extract with rapid qualitative filter paper, discard the first 2mL-3mL filtrate, and collect the subsequent filtrate for analysis.

[0065] 2) Preparation of reaction reagents

[0066] The preparation of buffer solution A is as follows: Weigh 71.6 grams of disodium hydrogen phosphate (12 crystal waters) and 15.2 grams of trisodium phosphate (12 crystal waters) into a beaker, dissolve them in water, then transfer them to a 1L volumetric flask, and dilute with water. To the scale.

[0067] The preparation of buffer solution B is: weigh 7g p-aminobenz...

Embodiment 2

[0092] Example 2 : Determination of total plant alkaloids in Burley tobacco

[0093] 1) Sample pretreatment

[0094] Prepare a sample of Burley tobacco powder according to YC / T31, and measure its moisture content, then weigh 0.25g of Burley tobacco sample in a 50mL conical flask with a stopper, add 25mL of 5% acetic acid, cover the stopper, and shake Shake on the device (rotational speed > 150rpm) and extract for 30min. Filter the extract with rapid qualitative filter paper, discard the first 2mL-3mL filtrate, and collect the subsequent filtrate for analysis.

[0095] 2) Preparation of reaction reagents

[0096] The preparation of buffer solution A is as follows: Weigh 71.6 grams of disodium hydrogen phosphate (12 crystal waters) and 15.2 grams of trisodium phosphate (12 crystal waters) into a beaker, dissolve them in water, then transfer them to a 1L volumetric flask, and dilute with water. To the scale.

[0097] The preparation of buffer solution B is: weigh 7g p-amino...

Embodiment 3

[0122] Example 3 : Determination of total plant alkaloids in cigarettes

[0123] 1) Sample pretreatment

[0124] Prepare cigarette shredded tobacco as a sample according to YC / T31, and measure its moisture content, then weigh 0.25g of the cigarette sample into a 50mL Erlenmeyer flask with a stopper, add 25mL of 5% acetic acid, cover the stopper, and oscillate on a shaker (speed >150rpm) extraction for 30min. Filter the extract with rapid qualitative filter paper, discard the first 2mL-3mL filtrate, and collect the subsequent filtrate for analysis.

[0125] 2) Preparation of reaction reagents

[0126] The preparation of buffer solution A is as follows: Weigh 83.0 grams of disodium hydrogen phosphate (12 crystal waters) and 20.0 grams of trisodium phosphate (12 crystal waters) into a beaker, dissolve them in water, then transfer them to a 1L volumetric flask, and dilute with water. To the scale.

[0127] The preparation of buffer solution B is: weigh 7g of p-aminobenzenesu...

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Abstract

The invention provides a buffer system suitable for a continuous flow method for measuring total alkaloid in tobacco and tobacco products. The buffer system comprises a buffer solution A and a buffer solution B; wherein the buffer solution A is prepared by the following steps: weighing 60.5 to 83.0 grams of disodium hydrogen phosphate (the preferable amount is 71.6 grams) and 10.1 to 20.2 grams of trisodium phosphate (the preferable amount is 15.2 grams), placing the weighed substances into a beaker, dissolving the mixture with water, then transferring the solution into a volumetric flask, and then filling water into the volumetric flask until the water reaches the scale; and the buffer solution B is prepared by the following steps: weighing 5.5 to 8.0 grams of p-aminobenzene sulfonic acid (the preferable amount is 7.0 grams), 60.5 to 83.0 grams of disodium hydrogen phosphate (the preferable amount is 71.6 grams), 5.0 to 7.0 grams of sodium dihydrogen phosphate (the preferable amount is 6.2 grams), and 10.0 to 12.1 grams of sodium citrate (the preferable amount is 11.8 grams), placing the weighed substances into a beaker, dissolving the mixture with water, transferring the solution into a volumetric flask, and then filling water into the volumetric flask until the water reaches the scale.

Description

technical field [0001] The invention belongs to the technical field of tobacco chemical component detection, and in particular relates to a buffer system suitable for continuous flow method determination of total alkaloids in tobacco and tobacco products. Background technique [0002] Nicotine is an important component of tobacco, and without nicotine, it cannot be called tobacco. In the routine analysis of tobacco, the content information of nicotine is usually obtained by measuring the content of total plant alkaloids in tobacco. The standards currently issued by the tobacco industry on the determination of total alkaloids in tobacco include: GB / T23225-2008 "Determination of total alkaloids in tobacco and tobacco products by photometry", YC / T160-2002 "Determination of total alkaloids in tobacco and tobacco products Continuous flow method" and YC / T468-2013 "Continuous flow (potassium thiocyanate) method for the determination of total plant alkaloids in tobacco and tobacco ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N21/75
Inventor 张威胡清源邢军孟庆华侯宏卫罗安娜刘楠何声宝王英元冯晓民王颖
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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