A method for detecting silver nanoparticles by fluorescence method based on g-tetramer

A technology of nano-silver particles and tetramers, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of distinguishing or detecting the total amount of nano-silver particles, and achieve stable and accurate results, great application prospects, and simple synthesis. Effect

Active Publication Date: 2016-08-17
安徽科博产品检测研究院有限公司
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Problems solved by technology

However, the total amount of silver nanoparticles in the sample cannot be directly distinguished or detected by G tetramer

Method used

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  • A method for detecting silver nanoparticles by fluorescence method based on g-tetramer

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Embodiment 1

[0027] A kind of method based on the fluorescence method of G tetramer to detect nano-silver particle, comprises the steps:

[0028] (1) Configure the sample solution to be tested: add 2.5 μL of 100 μM phosphoric acid and 5.7 μL of 88 mM hydrogen peroxide to the nano-silver aqueous solution. After adding, the total volume of the solution is 250 μL, so that H 3 PO 4 and H 2 o 2 The final concentrations were 1 μM and 2 mM respectively, and after mixing, react at room temperature for 30 min to obtain the sample solution to be detected;

[0029] (2) Configure multiple standard solutions: configure seven silver nitrate solutions with concentrations of 0.5, 1, 2, 4, 6, 8, and 10 μM respectively as seven standard solutions to draw a standard curve;

[0030] (3) Take eight 2 μL 100 μM single-stranded DNA (G-DNA) solutions containing G base repeat sequence, the G-DNA sequence is TGGGTAGGGCGGGTTGGGAAA, respectively add to eight 248 μL 20mM Tris-Ac, 2mM EDTA, pH 8.0 solution, heated ...

Embodiment 2

[0035] A kind of method based on the fluorescence method of G tetramer to detect nano-silver particle, comprises the steps:

[0036] (1) Configure the sample solution to be tested: add 10 μL of 100 μM phosphoric acid and 20 μL of 100 mM hydrogen peroxide to the nano-silver aqueous solution. 3 PO 4 and H 2 o 2 The final concentrations are 1μM and 2mM respectively, and after mixing, react at room temperature for 30min to obtain the sample solution to be detected;

[0037] (2) Configure multiple standard solutions: configure seven silver nitrate solutions with concentrations of 0.5, 1, 2, 4, 6, 8, and 10 μM respectively as seven standard solutions to draw a standard curve;

[0038](3) Take eight 8 μL 100 μM single-stranded DNA (G-DNA) solutions containing G base repeat sequence, the G-DNA sequence is TGGGTAGGGCGGGTTGGGAAA, and add them to eight 992 μL 20mM Tris-Ac, 2mM EDTA, pH 8.0 solution, heated at 90°C for 5 minutes to denature, mixed with equal volumes of sample solution...

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Abstract

The invention discloses a method for detecting nano-silver particles based on the fluorescence method of G tetramer. First, hydrogen peroxide is used to convert silver nanoparticles into monovalent silver ions in an acidic environment to obtain a sample solution to be detected; A variety of silver ion solutions are used as standard solutions to draw a standard curve; G-DNA is configured and mixed with equal volumes of sample solutions and standard solutions to make them fully interact; then potassium ions are added to make G-DNA fold into G tetramer structure; finally add N-methylporphyrin dipropionic acid IX (NMM), and measure the content of nano-silver in the sample solution by fluorescence method. The invention adopts the fluorescence method to quantitatively detect the nano-silver, which has the advantages of high sensitivity, simple operation and short time; the medium used in the detection is G-DNA, which has the characteristics of simple synthesis, low cost, good chemical stability and the like.

Description

technical field [0001] The invention relates to the technical field of environmental detection, in particular to a method for detecting nano-silver particles based on a G tetramer-based fluorescence method. Background technique [0002] Nano-silver is a metal silver element prepared with a particle size of 1-100nm, and its particle size is mostly around 20nm. It has a broad-spectrum bactericidal effect and does not produce drug resistance. , daily necessities and cosmetics, agricultural production, etc.) are widely used. The mass production and use of artificial nano silver particles will inevitably lead to its entry into the environment and its diffusion in the environment. However, a large number of studies have shown that nano-silver may have potential negative effects on the ecological environment and human health. For example, nano-silver can easily seep into wastewater during washing, thereby destroying the beneficial bacteria used in treatment plants to treat wastew...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 徐升敏陈少鹏吴李君
Owner 安徽科博产品检测研究院有限公司
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