Method for rapidly detecting amylase value in honey

Abstract

The invention discloses a method for rapidly detecting the amylase value in honey. The method comprises the following steps: 1, sample preparation: accurately weighing 5.0g of a honey sample, putting the honey sample in a beaker, adding 7.5mL of an enzymatic hydrolysis buffer solution and 10mL of distilled water, stirring until the sample is completely dissolved, transferring the above obtained solution to a 25mL volumetric flask, adding distilled water to a constant volume, and shaking up; 2, enzymatic hydrolysis: accurately sucking 2.5mL of the prepared sample solution to a test tube with 12.5mL of a starch solution, uniformly mixing, and carrying out enzymatic hydrolysis at normal temperature for 10min or 20min; 3, coloration: sucking the obtained enzymatic hydrolysis liquid to a colorimetric pipe with a coloration solution and distilled water, and coloring at normal temperature for 5min; and 4, detection: comparing the color of the coloration solution with a multicolor colourimetric card, and determining the amylase value in the honey. The method is characterized in that amylase reacts with starch at normal temperature, and a colourimetric card technique is cooperated to detect. The method has the advantages of simple operation, simple conditions, rapidness and the like.

Description

technical field [0001] The invention specifically relates to a rapid detection method for amylase value in honey, and belongs to the technical field of food quality and safety detection. Background technique [0002] The amylase value is one of the important quality indicators to measure the biological activity, maturity and freshness of honey. At present, the methods for determining the amylase value in honey mainly include spectrophotometer method, test tube visual method, standard reference color plate method and kit method. The first method is an internationally common method, which is accurate and fast, but requires spectrophotometers and other equipment, and has high technical requirements for testing personnel, and is not suitable for rapid on-site detection; the second method has complicated detection steps and is time-consuming The third method is a color comparison card developed on the basis of the first method, which is more convenient, but only has one color. F...

AI Technical Summary

Problems solved by technology

The first method is an internationally common method, which is accurate and fast, but requires spectrophotometers and other equipment, and has high technical requirements for testing personnel, and is not suitable for rapid on-site detection; the second method has complicated detection steps and is time-consuming The third method is a color comparison card developed on the basis of the first method, which is more convenient, but only has one color. For samples with low enzyme values, it needs to be detected for more than one hour. Spectrophotometry

In addition, none of the above methods can be detected at room temperature (enzymatic hydrolysis in a water bath at 40°C is required)