Rebaudioside M purification method

A technology of stevioside and purification method, which is applied in the field of extracting active ingredients, can solve problems such as low yield, defective purification process, unstable product quality, etc., achieve good taste and quality, and be beneficial to industrial production

Inactive Publication Date: 2014-11-19
QINGDAO RUNDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the steviol glycoside products on the market are mainly RA and STV, and RM is an ideal natural sweetness additive because of its good taste and taste and no bad aftertaste. In the purification method, there are still problems to be solved such as the defective purification process leading to unstable product quality and low yield

Method used

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  • Rebaudioside M purification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Get mother liquor sugar, the content of RM recorded through liquid chromatography analysis is 2.3%. The mother liquor sugar was made into a feed liquid with a concentration of 25g / L, and 1500L of feed liquid was taken out, and flowed slowly through a 500L DA-201-H macroporous adsorption resin column at a flow rate of 2.5L / min. During the process of the feed liquid flowing through the resin column, the resin column selectively adsorbs the feed liquid according to the polarity of different components of stevioside, and the pH of the adsorption environment is 5.5. After 10 hours, when the feed liquid adsorption is completed, use 1500L 77% ethanol resolves the steviol glycosides adsorbed on the resin. The eluate was intercepted in sections with 100 L as the unit, and the content of RM in the eluate was detected by liquid chromatography analysis. The test results found that stevioside RM began to be eluted in a large amount at 800L of the eluent, and the parameters of the m...

Embodiment 2

[0030] Take the mixed solvent that 10 kg of crude steviol glycosides with an RM content of 28.30% obtained in Example 1 and 30 kg of methanol with a mass concentration of 92% and 86% isopropanol are mixed in a ratio of 3:2 Mix, stir and dissolve at 65°C, quickly lower the solution to room temperature within 25 minutes, stir the mixture for 10-20 minutes every 4 hours, and after standing for 48 hours, separate the crystal mixture from solid to liquid to obtain an RM content of 80 % above crystals. After secondary crystallization, the obtained crystal content is above 95%. The filtered crystalline solid was dissolved in anhydrous salt, and the solution concentration was adjusted to 27%, then concentrated to about 43%, and the solution was dried to obtain 2.0 kg of refined stevioside, the refined stevioside product. Stevioside RM The content is 95.73%;

Embodiment 3

[0032]Take 10 kg of crude steviol glycosides with an RM content of 29.05% obtained in Example 1 and mix them with 32 kg of 90% methanol and 87% isopropanol in a ratio of 3:2. , after dissolution at 67°C, the solution was rapidly cooled to room temperature within 25 minutes, and the mixture was stirred for 10-20 minutes every 5 hours. After standing for 60 hours, the crystal mixture was subjected to solid-liquid separation through a plate and frame filter press to obtain crystals with an RM content of more than 85%. After secondary crystallization, the obtained crystal content is above 95%. The filtered crystalline solid was added into anhydrous salt water, and the concentration of the solution was adjusted to about 25%, and then concentrated to about 45%, and the solution was dried to obtain 2.2 kg of refined steviol glycosides. The stevioside RM content in the refined stevioside product is 95.34%;

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Abstract

The invention relates to a rebaudioside M purification method with the stages of crude extraction and subsequent refining treatment. The crude extraction stage comprises the following steps: a feed solution flows through eight macroporous absorption resin columns connected in series, and is absorbed sequentially; staged ethanol desorption is carried out, and analysis is carried out; an eluent is subjected to reduced-pressure concentration under high temperature; and an obtained solid is subjected to vacuum drying, such that crude rebaudioside M is obtained, wherein a crude rebaudioside M mass content reaches 25-35%. The subsequent refining treatment comprises the following steps: a mixed solvent is heated; the crude rebaudioside M is dissolved into the mixed solvent, such that a solution is formed; the temperature is reduced to normal temperature; standing and intermittent stirring is carried out; the product is allowed to stand for several hours, and solid-liquid separation is carried out; and the obtained solid is dried, such that refined rebaudioside M is obtained. The refined rebaudioside M content can be higher than 95%. The sweetness of the rebaudioside M is more than 300 times that of cane sugar. The sweetness is more long-lasting than that of cane sugar, and causes no bitter after-taste. The mouthfeel is closer to white sugar, and mouthfeel and taste quality are good. Rebaudioside M is an ideal natural sweet additive.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from natural plants, in particular to a method for purifying stevioside RM (Rebaudioside M) from stevia rebaudiana with high yield. Background technique [0002] Stevia extract is widely used in food, beverage, wine making, medicine, cosmetics and other fields. In recent years, stevioside, the extract of stevia rebaudiana, has been used as a sweetener. It has very low calories and will not cause adverse reactions to patients with dental diseases and diabetes. Stevioside is an excellent sweetener, which has broad market prospects in food and beverage applications. Stevioside is the collective name for the extract of stevia rebaudiana. At present, in the official documents of the US FDA, stevioside mainly involves the following components: Stevioside (stevioside), Steviolbioside (steviobioside), Rubusoside ( rubusoside), Dulcoside A (Dulcoside A), Rebaudioside A (rebaudioside A), Rebau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08
CPCA23L2/60A61K36/28A61K2236/00A23L27/36B01D15/361C07H1/08C07H15/256A23V2002/00A61K47/26A23L33/105
Inventor 徐兴光张永李存彪
Owner QINGDAO RUNDE BIOTECH
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