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Heterotrophic nitrification bacterial strain

A heterotrophic nitrification and bacterial technology, applied in the field of microorganisms, can solve the problems of low total nitrogen removal rate, weak impact resistance, slow proliferation of autotrophic bacteria, etc., and achieve a strong degradation effect

Inactive Publication Date: 2014-11-19
JINGGANGSHAN UNIVERSITY
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Problems solved by technology

Autotrophic bacteria proliferate slowly and it is difficult to maintain a high biological concentration. Aeration treatment is required to reduce the concentration of organic matter before the strain can grow and exhibit biological activity, and the impact resistance is weak; high concentrations of ammonia nitrogen and nitrite will inhibit nitrification The growth of bacteria makes the nitrification incomplete, resulting in a low removal rate of total nitrogen

Method used

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  • Heterotrophic nitrification bacterial strain

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Embodiment Construction

[0017] 1. Isolation of strain HLNR02

[0018] (1) Collect water samples from Jiangjun Lake, Ji’an County, Ji’an City, Jiangxi Province, filter 1L of the water in the water samples with a 0.22μm filter membrane, rinse the filter membrane with 10mL of sterile water, and take 1mL of the supernatant after algae precipitation In 99 mL of sterilized ammonium nitrogen-containing enrichment medium, the medium was placed in a shaking incubator at a temperature of 28° C. and a vibration frequency of 200 rpm / min for 4 days. After 4 days, take 1 mL of the bacterial liquid and put it in 99 mL of sterilized ammonium nitrogen-containing enrichment medium, and culture it under the above-mentioned culture conditions for 4 days. The same operation was repeated 3 times for 4 cycles of enrichment culture.

[0019] (2) Take 1 mL of the bacterial liquid obtained from enrichment in step (1) to the 4th cycle, add it to the acclimatization medium with the concentration of ammonia nitrogen increased b...

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Abstract

The invention discloses a heterotrophic nitrification bacterial strain which is named as Klebsiella sp.HLNR02 and is collected in the China General Microbiological Culture Collection Center (CGMCC) in 3#, No.1 Courtyard, West Beichen Road, Chaoyang District, Beijin in October, 2013, and the collection number is CGMCC No.8397. The heterotrophic nitrification bacterial strain has the beneficial effects that the heterotrophic nitrification bacterial strain is relatively high in degradation effect on ammonia nitrogen and total nitrogen in a eutrophic water body, and a good microorganism material is provided for biological treatment on a water body polluted by ammonia nitrogen wastewater by further using the stain.

Description

Technical field: [0001] The invention relates to a bacterial strain in the field of microorganisms, in particular to a heterotrophic nitrifying bacterial strain. Background technique: [0002] The transformation of nitrogen form is closely related to the nitrogen removal efficiency of sewage sludge, the improvement of nitrogen removal process, and the solution of water eutrophication. At present, there are physical, chemical and biological methods to solve the nitrogen eutrophication of water bodies. With the aggravation of nitrogen pollution and people's increasing attention to environmental issues, nitrogen removal technology, especially biological nitrogen removal technology has become an important direction and means to control water pollution. [0003] Ammonia nitrogen is one of the pollutants that cause eutrophication of water bodies. At present, it is generally believed that in the process of biological denitrification, ammonia nitrogen in wastewater is first oxidiz...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/22
Inventor 贺根和张宇燕梁亮亮李一弘
Owner JINGGANGSHAN UNIVERSITY
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