Method for establishing pathogenic bacterium fingerprint by using Terahertz waves

A fingerprint and terahertz technology, applied in the field of detection, can solve the problems of no fingerprint of pathogenic bacteria, no detection of liquid samples, etc., and achieve the effect of simple method

Inactive Publication Date: 2014-11-19
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, terahertz waves can only be used to detect solid samples, and there are no reports on liquid samples, let alone the fingerprints of pathogenic bacteria in liquid samples.

Method used

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  • Method for establishing pathogenic bacterium fingerprint by using Terahertz waves
  • Method for establishing pathogenic bacterium fingerprint by using Terahertz waves

Examples

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Effect test

Embodiment 1

[0015] The method for establishing the fingerprint of pathogenic bacteria by using terahertz waves, the specific steps are as follows:

[0016] a. Take the Escherichia coli that has been identified as freeze-thawed at room temperature (18-25°C) and inoculate it into LB (Luria-Bertani) liquid medium with an inoculation loop, and then culture overnight at 37°C to the concentration of the bacterial solution Greater than 0.5 McFarland units (approximately 1.5×10 8 cfu / mL);

[0017] b. Centrifuge the bacterial solution cultivated in step a at 1500g for 5min, discard the supernatant, add normal saline to the precipitate, mix well and then centrifuge at 1500g for 5min; then discard the supernatant, add normal saline to shake and mix well Until the turbidity of the bacterial solution is 0.5 McFarland units;

[0018] c. Detect the bacteria liquid obtained in step c at a terahertz frequency of 0.2-3 THz, a wavelength of 100-1500 μm, an average wavelength of 300 μm, nitrogen flushing, ...

Embodiment 2

[0020] The method for establishing the fingerprint of pathogenic bacteria by using terahertz waves, the specific steps are as follows:

[0021] a. Take the identified Staphylococcus aureus, freeze-thaw at room temperature (18-25°C), inoculate it into LB (Luria-Bertani) liquid medium with an inoculation loop, and then culture overnight at 37°C to the concentration of the bacterial solution Greater than 0.5 McFarland units (approximately 1.5×10 8 cfu / mL);

[0022] b. Centrifuge the bacterial solution cultivated in step a at 3000g for 10min, discard the supernatant, add normal saline to the precipitate, mix well and then centrifuge at 3000g for 10min; then discard the supernatant, add normal saline to shake and mix well Until the turbidity of the bacterial solution is 0.5 McFarland units;

[0023] c. Detect the bacteria liquid obtained in step c at a terahertz frequency of 0.2-3 THz, a wavelength of 100-1500 μm, an average wavelength of 300 μm, nitrogen flushing, ambient humidi...

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Abstract

The invention discloses a method for establishing pathogenic bacterium fingerprint by using Terahertz waves. The method comprises the following concrete steps: subjecting a to-be-detected bacterial strain to enrichment culture, carrying out cleaning with normal saline and collection and adjusting turbidity of a bacterial liquid to 0.5 McFarland (MCF) by using a bacterial nephelometer or turbidity meter; and then carrying out detection under the conditions that Terahertz frequency is 0.2 to 3 THz, wavelength is 100 to 1500 mu m, average wavelength is 300 mu m, nitrogen is used for flushing, ambient humidity is less than 5%, and ambient temperature is 22 DEG C. The method is simple and fast, can detect liquid samples and is capable of identifying the variety of the to-be-detected bacterial strain according to the established fingerprint.

Description

technical field [0001] The invention belongs to the field of detection, and in particular relates to a method for establishing the fingerprint of pathogenic bacteria by using terahertz waves. Background technique [0002] Spectroscopy plays a very important role in the research and analysis of biomolecules such as carbohydrates, proteins, and nucleic acids. Terahertz Waves (THz) waves refer to electromagnetic waves with a frequency between 0.1 and 10 THz (1 THz = 1012 Hz) (wavelength between 30 and 3000 μm), and its wave band is between microwave and infrared light. The rapid development of ultrafast laser technology and semiconductor material science and technology in the past decade has provided a stable and reliable excitation source for the generation of THz pulses, and promoted the application of THz radiation in spectroscopy and imaging technology. [0003] Compared with traditional techniques such as ultraviolet-visible absorption spectroscopy and X-ray imaging analy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/14C12Q1/10C12R1/19C12R1/445
Inventor 府伟灵黄庆张立群杨翔李永川王云霞
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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