VP7 gene-based bovine rotavirus qPCR detecting kit

A detection kit, bovine rotavirus technology, applied in the biological field, can solve problems such as no bovine rotavirus diagnosis

Inactive Publication Date: 2014-11-19
魏锁成 +2
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no specific kit for the diagnosis of bovine rotavirus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • VP7 gene-based bovine rotavirus qPCR detecting kit
  • VP7 gene-based bovine rotavirus qPCR detecting kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Sample collection and processing

[0015] (1) Tissue sample processing. Each tissue was weighed about 1g sample from three different positions, cut and mixed with surgical scissors, then took 0.05g and ground it in a grinder, added 1.5 mL normal saline to continue grinding, and transferred to 1.5 mL after homogenization. Centrifuge at 8,000 rpm for 2 min, and take 100 μL of the supernatant into a 1.5 mL sterilized centrifuge tube.

[0016] (2) Whole blood sample processing. After hemagglutination, take 100 μL of serum and put it in a 1.5 mL sterilized centrifuge tube.

[0017] (3) Positive control treatment. Take 100 μL of the positive control and place it in a 1.5 mL sterilized centrifuge tube.

[0018] (4) Negative control treatment. Take 100 μL of the negative control and put it in a 1.5mL sterilized centrifuge tube.

[0019] RNA extraction

[0020] Take the processed sample, negative control and positive control, add 600 μL of lysate respectively, mix well by...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a VP7 gene-based bovine rotavirus qPCR detecting kit, comprising the components such as a specific primer of a conserved sequence of a VP7 gene, reverse transcriptase and heat-resistant DNA polymerase (Taq enzyme); the detected highest dilution degree can be up to 10<-5> to 10<-4>; the linear range of quantitative detection can be up to 10<-1> dupes/mu L; the VP7 gene based bovine rotavirus qPCR detecting kit does not have any amplification to other viral nucleic acids, bacteria and negative control groups; and the detecting kit is rapid, sensitive and specific and can be used for quantitatively detecting bovine rotavirus in various samples such as feces and blood.

Description

technical field [0001] The invention relates to a bovine rotavirus qPCR detection kit based on VP7 gene, belonging to the field of biotechnology. [0002] Background technique [0003] Rotavirus (rotavirus, RV) is an important cause of severe dehydrating diarrhea in infants and young animals worldwide. Rotavirus gastroenteritis is a widespread zoonotic disease, especially an acute intestinal infectious disease of young animals and infants. It is characterized by diarrhea and dehydration, and the case fatality rate can reach 50%. The genome of rotavirus consists of 11 discontinuous dsRNA fragments, encoding 6 structural proteins (VP1-VP4, VP6, VP7) and 5 non-structural proteins (NSP1-NSP5); VP7 and VP4 constitute the outer coat Shell, VP7 has serotype specificity, is related to pathogenicity, and can stimulate the body to produce protective antibodies. VP7 is an N-linked oligomannose glycoprotein, which is an important glycoprotein of the outer membrane of the virus and th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
CPCC12Q1/701C12Q1/6851C12Q1/70C12Q2531/113C12Q2561/113
Inventor 魏锁成郭慧琳车团结
Owner 魏锁成
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap