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Porcine leukocyte interferon freeze-dried powder injection and preparation method thereof

A technology of leukocyte interferon and freeze-dried powder injection, which is applied in the field of porcine leukocyte interferon freeze-dried powder injection and its preparation, can solve the problems of low safety of interferon, limited promotion, and insignificant effect, and achieve protein precipitation The effect of the phenomenon

Active Publication Date: 2016-05-11
SICHUAN SHIHONG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that interferon not only has antiviral effects, but also has antitumor effects, and a series of physiological activities such as regulating the immune system and anti-cells. However, in the field of veterinary medicine, animal interferon has not been widely used. application, the main reason is that the currently obtained animal interferon is not safe, the preparation process is complicated, the cost is high, the price is expensive, the effect is not significant, and the promotion is limited

Method used

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  • Porcine leukocyte interferon freeze-dried powder injection and preparation method thereof
  • Porcine leukocyte interferon freeze-dried powder injection and preparation method thereof
  • Porcine leukocyte interferon freeze-dried powder injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 porcine leukocyte interferon freeze-dried powder injection and preparation method thereof

[0043] The preparation method of the porcine leukocyte interferon freeze-dried powder injection of the present embodiment comprises the following steps:

[0044] Step 1, preparation of porcine leukocyte interferon stock solution

[0045] 1. Preparation of porcine white blood cell suspension: Aseptically collect fresh blood from healthy pigs, centrifuge at 4°C at 3000 rpm for 20 minutes under sterile conditions, first suck out the plasma, and then suck the gray-yellow middle layer of white blood cells into the plasma bottle within, with 0.85% NH 4 Centrifuge after Cl treatment twice, collect precipitated white blood cells, and wash away residual NH with Hank's solution 4 Cl, dilute the precipitated white blood cells into a suspension with culture medium.

[0046]2. Preparation of pig serum: Aseptically collect healthy pig blood, separate out naturally, and use 0.2%...

Embodiment 2

[0066] Embodiment 2 porcine leukocyte interferon freeze-dried powder injection and preparation method thereof

[0067] The preparation method of the porcine leukocyte interferon freeze-dried powder injection of the present embodiment comprises the following steps:

[0068] Step 1, preparation of porcine leukocyte interferon stock solution

[0069] 1. Preparation of porcine white blood cell suspension: Aseptically collect fresh blood from healthy pigs, centrifuge at 4°C at 3000 rpm for 20 minutes under sterile conditions, first suck out the plasma, and then suck the gray-yellow middle layer of white blood cells into the plasma bottle within, with 0.85% NH 4 Centrifuge after Cl treatment twice, collect precipitated white blood cells, and wash away residual NH with Hank's solution 4 Cl, dilute the precipitated white blood cells into a suspension with culture medium.

[0070] 2. Preparation of pig serum: Aseptically collect healthy pig blood, separate out naturally, and use 0.2...

Embodiment 3

[0086] Embodiment 3 field control treatment test and field extension treatment test

[0087] The porcine leukocyte interferon freeze-dried powder injection prepared in Example 1, as a broad-spectrum antiviral drug, has a significant effect on the treatment of porcine viral diseases, and a total of 9843 cases of viral diseases were treated (Table 1).

[0088] The curative effect of porcine leukocyte interferon freeze-dried powder injection of the present invention in the treatment of porcine viral diseases of table 1

[0089] curative effect

Number of cases

cure rate

cure

9308

94.57％

[0090] Among them, the effective rate of treating suspected swine fever is 85.1%, and the cure rate is 83.47%; the effective rate of treating swine viral diarrhea is 99.95%, and the cure rate is 95.81%; the effective rate of treating piglet pullorum is 100%, and the cure rate is 98.3%.

[0091] The cure rate of treating porcine viral influenza is 100%.

[...

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Abstract

The invention provides a swine leukocyte interferon freeze-dried powder injection and a preparation method thereof. The freeze-dried powder injection is prepared by mixing and freeze-drying a swine leukocyte interferon original liquid and a protective agent according to a volume ratio of 20:4-3, wherein the original liquid comprises the following components: a swine leukocyte suspension, swine serum, a starting interferon, a sendai virus liquid and a culture liquid. The preparation method comprises the processes of raw material preparation, mixing, water bath stirring induced culture, centrifugation harvesting, virus inactivation, thawing sterilization, protecting agent addition, and freeze-drying. The injection has the function of being used as a broad-spectrum antiviral drug for treating swine fever, swine viral diarrhea, swine viral cold and other viral diseases. The total cure rate of field control and promotion treatment tests is 94.57%, and the total cure rates of artificial control treatment of swine fever and swine viral diarrhea are respectively 83.3% and 100%.

Description

technical field [0001] The invention relates to a porcine leukocyte interferon freeze-dried powder injection and a preparation method thereof. Background technique [0002] Porcine viral disease is the most serious disease that harms the pig industry in porcine infectious diseases. Among porcine viral diseases, swine fever is the most harmful. Its morbidity and mortality are very high, and there is no effective treatment. At present, the occurrence of porcine virulent diseases is mainly controlled by means of epidemic prevention. In recent years, studies have found that interferon not only has antiviral effects, but also has antitumor effects, and a series of physiological activities such as regulating the immune system and anti-cells. However, in the field of veterinary medicine, animal interferon has not been widely used. Application, the main reason is that the safety of animal interferon obtained at present is not high, the preparation process is complicated, the cost i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/21A61K9/19A61P31/12A61P31/16A61K35/14
Inventor 荣政
Owner SICHUAN SHIHONG BIOLOGICAL TECH