Sika deer mucosal disease homologous inactivated vaccine and preparation method thereof

A technology for inactivated vaccines and mucosal diseases, which is applied in the direction of antiviral agents, virus antigen components, emulsion delivery, etc., can solve the problems of no immunity, etc., and achieve the effect of simple emulsification procedure and suitable viscosity

Inactive Publication Date: 2014-12-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the bottleneck problem that restricts the rapid development of the sika deer industry is that the special vaccine for sika deer mucous membrane disease is still blank in my country, and most deer farms are in a state of no immunity

Method used

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  • Sika deer mucosal disease homologous inactivated vaccine and preparation method thereof
  • Sika deer mucosal disease homologous inactivated vaccine and preparation method thereof
  • Sika deer mucosal disease homologous inactivated vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, sika deer mucosal disease homologous inactivated vaccine and preparation method thereof

[0021] In order to safely and specifically prevent the viral infectious disease of sika deer mucosal disease, a homologous inactivated vaccine was developed. Its preparation method is as follows:

[0022] 1. Preparation of sika deer mucosal disease virus solution

[0023] 1. Materials

[0024] Sika deer-derived bovine viral diarrhea strain CC-CRB01 (isolated and identified by the Laboratory of Infectious Diseases, College of Veterinary Medicine, Jilin University) is the second-generation basic virus, with a virulence of 10 5.5 TCID 50 / 0.1mL

[0025] Determination of CC-CRB01 Strain Virus Content

[0026] Dilute the virus sample 10 times, and inoculate it on the MDBK cells that have grown into a single layer, repeat 4 wells for each dilution, and use the well directly added to the maintenance solution as a blank control, 37 ° C, 5% CO 2 After culturing for 1 h, ...

Embodiment 2

[0090] Embodiment 2, the detection of the effect of the homologous inactivated vaccine of sika deer mucosa disease

[0091] 1. Field trial report of homologous inactivated vaccine against sika deer mucosal disease

[0092] After completing the laboratory test of the inactivated vaccine, field tests were carried out with four batches of qualified laboratory products to observe the safety and efficacy of the vaccine under production conditions.

[0093] 1. Materials

[0094] Vaccine: the homologous inactivated vaccine of sika deer mucosal disease with batch numbers 00201, 00202, 00203, and 00301 prepared in Example 1.

[0095] Immune doe: select 4 test sites are:

[0096]

[0097] Clinical examination before immunization Observe the performance and remove the diseased deer.

[0098] 2. Field efficacy test

[0099] 1) Immunization: Batch numbers 00201, 00202, 00203, and 00301 sika deer mucosal disease homologous inactivated vaccines prepared in Example 1 were intramuscular...

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Abstract

The invention discloses a sika deer mucosal disease homologous inactivated vaccine and a preparation method thereof. The preparation method comprises the following steps: performing inoculated culture on deer source BVDV (bovine viral diarrhea / virus) to the third to sixth generations in stable cell flasks with MDBK (madin-darby bovine kidney) cells fully spread in a single layer, selecting cell flasks which are cultured for 72-144 hours and generate a cytopathic effect (CPE), repeatedly freezing and thawing after pathological change, collecting viral liquid, adding formaldehyde into the viral liquid for inactivating, mixing the inactivated viral liquid with an oil agent consisting of white oil and sorbitan, and adding aluminum stearate to obtain the sika deer mucosal disease homologous inactivated vaccine.

Description

technical field [0001] The invention relates to a homologous inactivated vaccine for sika deer mucosal disease and a preparation method thereof. Background technique [0002] Sika deer mucosal disease is caused by bovine viral diarrhea / mucosal disease virus (BVDV) and is characterized by fever, mucosal erosion and ulceration, leukopenia, diarrhea, immune tolerance and persistent infection, immunosuppression, congenital defects, cough, pregnant doe A contagious disease characterized by miscarriage, stillbirth or deformed fetus seriously affects the healthy development of the deer industry. At present, the bottleneck problem that restricts the rapid development of the sika deer industry is that there is still no vaccine for sika deer mucosal diseases in my country, and most deer farms are in a state of no immunity. [0003] Inactivated vaccine refers to the use of physical or chemical methods to make pathogenic organisms and their metabolites lose their virulence, but still r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K9/107A61P31/14
Inventor 丁壮董航丛彦龙尹仁福母连志张晓东周玉龙穆昱
Owner JILIN UNIV
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