Method for determining pollen viability of watermelon

A technology for watermelon pollen and vitality, which is applied to measuring devices, plant cells, instruments, etc., to achieve the effects of simple detection method, uniform and stable medium composition, and accurate data

Inactive Publication Date: 2014-12-03
ZHENJIANG SUIHAN AGRI
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Problems solved by technology

Ledue et al. used the improved Monnier medium ME3 as the germination medium to significantly increase the germination rate of shepherd's purse (trinucleated pollen) pollen (LeducN, MonnierM, DouglasGC. Germination of trinucleated pollen: formulation of a new medium for Capsellabursa-pastoris[J]. SexualPlantReproduction, 1990,3:228‐235.) In addition, most of the pollen germination assays in vitro use liquid culture. Although the culture medium can fully contact the pollen surface, the nutrient substances tend to be unevenly distributed and ventilated if the standing time is too long. Poor resistance, which greatly affects the normal germination of pollen and distorts the measurement results
[0003] At present, there are no reports about the culture medium and culture conditions of in vitro germination of watermelon pollen, and the present invention can provide the most suitable pollen in vitro germination culture system for watermelon varieties , to provide an efficient, fast and simple method for pollen viability determination

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  • Method for determining pollen viability of watermelon
  • Method for determining pollen viability of watermelon

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specific Embodiment

[0027] The present embodiment measures the vigor of watermelon variety 'wmj01' pollen by the following method:

[0028] Step 1, preparation medium, composition includes 200g / L sucrose, 10mg / LH 3 BO 3 , 800mg / LCa (NO 3 ) 2 4H 2 O, and 6.5g / L agar, the pH value is 5.8;

[0029] Step 2, collect pollen, collect pollen of watermelon in full bloom at 9-11 in the morning, put the pollen into a centrifuge tube, and store it away from light;

[0030] Step 3, cultivating pollen, sowing the pollen evenly on the solid medium, and culturing for 4 hours under dark conditions at 25°C and 85% relative humidity;

[0031] Step 4: Microscopic observation and calculation of pollen viability. After the cultivation is completed, put it under an optical microscope to observe the germination status. The pollen tube length is greater than the pollen diameter as pollen germination. Randomly observe and record 10 fields of view. The experiment is repeated 3 times, and the results are shown in figu...

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Abstract

The invention relates to a method for determining the pollen viability of watermelon, belonging to the technical field of plant. The invention discloses a method for determining the pollen viability of watermelon by using an in-vitro germination method, and the pollen viability of the watermelon can be effectively determined. An in-vitro pollen germination culture medium used comprises the following components of 200g / L sucrose, 10mg / L H3BO3, 800mg / L Ca(NO3)2.4H2O, and 6.5g / L agar. The method comprises the following steps: evenly sowing pollen into the solid culture medium, and culturing at dark conditions 85% relative humidity at 25DEG C; and after four hours, observing the pollen germination and pollen tube growth situations through an optical microscope. The method is simple, completely quantitative, reliable in data, and strong in operability, has higher practical application values, and achieves important significance on the breeding efficiency of watermelons.

Description

technical field [0001] The invention relates to a method for measuring pollen vigor, in particular to a method for measuring watermelon pollen vigor, and belongs to the technical field of plants. Background technique [0002] Watermelon (Citrullus lanatus Mansfeld) is a plant of the genus Watermelon in the family Cucurbitaceae. It is a widely planted worldwide horticultural crop and one of the favorite fruits. There are many methods for measuring pollen viability, among which pollen germination in vitro is the most effective and closest to actual results. Determination of pollen viability by in vitro germination requires specific medium and culture conditions. The main components of the medium generally include Ca2+, boron, carbon source, etc., and some plants also need other substances such as hormones (such as 6-BA, etc.), PEG, etc. Studies by Brewbaker et al. have shown that 39 families, 79 genera, and 86 species of plant pollen can germinate well on BK medium (Brewbake...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04G01N33/00
Inventor 孙春青潘跃平马志虎戴忠良毛忠良吴国平秦文斌姚悦梅张振超潘永飞孙国胜王建华陈智超
Owner ZHENJIANG SUIHAN AGRI
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