Liquid-phase hybrid capture enriching liquor for genome DNA sequencing library and hybrid method adopting liquid-phase hybrid capture enriching liquor

Abstract

The invention discloses a liquid-phase hybrid capture enriching liquor for a genome DNA sequencing library and a hybrid method adopting the liquid-phase hybrid capture enriching liquor. The hybrid enriching liquor comprises phosphate buffer, sodium citrate buffer (SSC), SDS (lauryl sodium sulfate), EDTA (ethylene diamine tetraacetic acid) and Denhardt liquor. The hybrid liquor and the corresponding hybrid method are low in cost, simple and convenient to implement; the hybrid time can be effectively shortened, the needed DNA fragment can be precisely extracted, influences of a background value on a signal value can be reduced, and the hybrid target enriching effects can be optimized.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a formulation and a hybridization method of a genomic DNA sequencing library liquid-phase hybridization trapping enrichment solution. Background technique [0002] In the past few years, DNA sequencing technology has undergone a fundamental shift, from traditional Sanger sequencing to the so-called "Next Generation Sequencing" technology (Next Generation Sequencing, NGS). Its outstanding feature is massively parallel sequencing. NGS technology allows millions or even hundreds of millions of sequencing reactions to be performed simultaneously, thereby achieving a huge increase in sequencing throughput. The NGS technology platform needs to construct a fragment library suitable for massively parallel sequencing. The process of constructing a sequencing library involves DNA fragmentation, subsequent DNA repair and end processing (blunt ends or A overhangs). Finally, th...

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However, its hybridization solutions and hybridization methods are mostly aimed at the capture probes produced by our com...

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