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Method for detecting or auxiliarily detecting Phytophthora bacteria

A Phytophthora genus and auxiliary detection technology, applied in the biological field, can solve the problems of high requirements for the culture environment, long time-consuming morphological identification methods, and low accuracy, and achieve the effect of accurate results, high accuracy, and simple methods

Inactive Publication Date: 2014-12-03
ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU +2
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Problems solved by technology

However, the morphological identification method takes a long time, is not accurate, and has high requirements for the culture environment

Method used

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  • Method for detecting or auxiliarily detecting Phytophthora bacteria
  • Method for detecting or auxiliarily detecting Phytophthora bacteria
  • Method for detecting or auxiliarily detecting Phytophthora bacteria

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example 1

[0027] The strain to be tested was donated by the National Key Laboratory of Plant Pathogenic Fungi of Nanjing Agricultural University and identified as Phytophthora capsici ( Phytophthora capsici ) strains, see the following references for the specific process of identification:

[0028] Zheng Xiaobo, Lu Jiayun. Distribution and mating types of Phytophthora species in East China and Northeast China. Journal of Nanjing Agricultural University, 1990, 13(3): 61-64.

[0029] Now adopt the method of the present invention to identify its species name.

[0030] (1) Genomic DNA of the strain to be tested was extracted, the ITS coding gene was amplified using universal primers (SEQ ID NO:5 and SEQ ID NO:6), and the amplified product was sequenced. The result is shown in SEQ ID NO:13.

[0031](2) Log in to the Phytophthora Database (http: / / www.phytophthoradb.org / ), and download the ITS coding genes of all Phytophthora (Phytophthora) bacteria. There are 143 ITS coding genes downloade...

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Abstract

The invention provides a method for detecting or auxiliarily detecting Phytophthora bacteria, relating to the technical field of biology. The method comprises the following steps: carrying out multiple sequence comparison on DNA (deoxyribonucleic acid) bar code sequences of Phytophthora bacteria, detecting specific base sites of each variety, and meanwhile, obtaining a consistency sequence, wherein the specific base sites of each variety comprise differentiated base sites and SNP (single-nucleotide polymorphism) sites; comparing the DNA bar code sequence of the bacterium to be detected with the consistency sequence, and regulating the length to obtain a positioning sequence; and comparing the positioning sequence with the specific base sites of each variety, marking the degree of similarity between the bacterium to be detected and various bases, taking the maximum value, and determining that the bacterium to be detected belongs to the variety with the maximum value. The method can identify the Phytophthora bacteria, is simple to operate, and has the advantages of high accuracy, short time consumption and favorable specificity.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting or assisting in detecting Phytophthora. Background technique [0002] Phytophthora ( Phytophthora de Bary) is a worldwide distribution of important plant pathogens, its genus is called Phytophthora ( Phytophthora ), Phytophthora comes from the Greek, Phyto means plant (plant), phthora means destroyer (destroyer), and most species of this genus are indeed seriously harmful and destructive to their host plants. Because Phytophthora is highly destructive and harmful to host plants, it often leads to serious losses of agricultural production and destruction of forest ecology, so plant diseases caused by Phytophthora are usually called "blight". All over the world, the annual agricultural losses caused by epidemic diseases are as high as tens of billions of dollars, and the annual losses caused by diseases in Australia alone reach more than 2 billion U.S. dollars...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04C12R1/645
CPCC12Q1/04C12Q1/6895C12Q2600/16
Inventor 粟寒张勇王振华杨静缪爱斌李彬吴翠萍
Owner ANIMAL AND PLANT & FOOD DETECTION CENTER JIANGSU ENTRY EXIT INSPECTION AND QUARANTINE BUREAU
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