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Kit and preparation method thereof

A technology of kits and reagents, applied in the fields of biology and medical testing, can solve the problems of not being able to meet the needs of cervical cancer screening, complex detection process, high reagent cost, etc. The effect of high positive rate and accuracy

Inactive Publication Date: 2014-12-03
CHENGDU XUHUA BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing detection methods have high cost of reagents, complex detection process, and long cycle, which cannot meet the needs of the current large-scale cervical cancer screening

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Make 500 kits

[0024] 1. The test reagent is distilled water, prepare 500ml of distilled water for use.

[0025] 2. The contrast agent solution is mercuric chloride solution, and 500ml of 0.02mol / l mercuric chloride solution is prepared.

[0026] 3. The chromogenic agent is a mixture of phosphotungstic acid reagent and buffer solution at a volume ratio of 1:1.

[0027] 1. Phosphotungstic acid reagent, weigh 41g of sodium tungstate, dissolve in 320ml of distilled water, add 35ml of 85% phosphoric acid, reflux and boil for 2 hours, cool to room temperature, add distilled water to make up to 1000ml; add 35g of lithium sulfate to dissolve, put in 2 Store in refrigerator at ℃-8℃ for later use.

[0028] 2. The pH of the buffer solution is 5.4. According to the requirements of the pH value, prepare 1000ml of sodium acetate / acetic acid buffer solution: mix 833.3ml of 2mol / L sodium acetate solution with 166.7ml of 2mol / L acetic acid solution, and store in a refrigerator at 2°...

Embodiment 2

[0032] Make 500 kits

[0033] 1. The test reagent is distilled water, prepare 500ml of distilled water for use.

[0034] 2. The contrast agent solution is mercuric chloride solution, and 500ml of 0.03mol / l mercuric chloride solution is prepared.

[0035] 3. The chromogenic agent is a mixture of phosphotungstic acid reagent and buffer solution at a volume ratio of 1:1.

[0036] 1. Phosphotungstic acid reagent, weigh 45g of sodium tungstate, dissolve in 350ml of distilled water, add 40ml of 85% phosphoric acid, reflux and boil for 2 hours, cool to room temperature, add distilled water to make up to 1000ml, add 35g of lithium sulfate to dissolve, set in 2 Store in refrigerator at ℃-8℃ for later use.

[0037] 2. The pH of the buffer solution is 5.7. According to the requirements of the pH value, prepare 1000ml of sodium dihydrogen phosphate / disodium hydrogen phosphate buffer solution: mix 935ml of 0.2mol / L sodium dihydrogen phosphate solution with 65ml of 0.2mol / L disodium hydro...

Embodiment 3

[0041] Make 1000 kits

[0042] 1. The test reagent is distilled water, and 1000ml of distilled water is prepared for use.

[0043] 2. The contrast agent solution is mercuric sulfate solution, and 1000ml of 0.02mol / l mercuric sulfate solution is prepared.

[0044] 3. The chromogenic agent is a mixture of phosphotungstic acid reagent and buffer solution at a volume ratio of 1:1.

[0045] 1. Phosphotungstic acid reagent, weigh 80g of sodium tungstate, dissolve in 700ml of distilled water, add 80ml of 85% phosphoric acid, reflux and boil for 2 hours, cool to room temperature, add distilled water to make up to 2000ml, add 65g of lithium sulfate to dissolve, set in 2 Store in refrigerator at ℃-8℃ for later use.

[0046] 2. The pH of the buffer solution is 5.8. According to the requirements of the pH value, prepare 2000 ml of sodium dihydrogen phosphate / disodium hydrogen phosphate buffer solution: mix 1840 ml of 0.2 mol / L sodium dihydrogen phosphate solution with 160 ml of 0.2 mol / ...

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Abstract

The invention discloses a kit for detecting sulfydryl metabolite in urine prompting the cervical lesion and a preparation method thereof. The kit comprises a reference agent, a testing agent and a color developing agent, wherein the reference agent is a mercuric chloride solution or a mercury sulfate solution, and the color developing agent is a phosphotungstic acid reagent; the kit is prepared in a sterility packaging manner. The remarkable increment of sulfydryl metabolite in female urine is proved to prompt the cervical lesion. The kit can detect the content of the sulfydryl metabolite at a preclinical phase and a clinical phase, the positive rate and accuracy for detecting the cervical cancer are high, the missed diagnosis can be avoided, a subject is prevented from being wounded and crossly infected, and instruments and equipment are not needed; the detection process is simple, the acceptance of the subject is high, the manpower and material resource can be greatly saved, workload of the examination personnel can be remarkably alleviated, the bottleneck that the screening number cannot reach the standard can be effectively broken through, and the kit is particularly suitable for surveying the cervical cancer in a wide range.

Description

technical field [0001] The invention relates to the fields of biology and medical examination, in particular to a detection kit for detecting sulfhydryl metabolites in urine for prompting cervical cancer lesions and a preparation method thereof. Background technique [0002] Common cervical cancer screening methods include HPV (human papillomavirus) detection, TCT (liquid-based thin layer cytology), Pap smear detection and pathological diagnosis. Existing detection methods have high cost of reagents, complex detection process, and long cycle, which cannot meet the needs of the current large-scale cervical cancer screening. [0003] With the development of biological technology, researchers have found that the level of sulfhydryl metabolites in women's urine is significantly increased, which can indicate the occurrence of cervical cancer. The detection of sulfhydryl metabolites can better predict the occurrence of cervical cancer in advance. This is because HPV (human papil...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 李再成李德海王奇杨涛
Owner CHENGDU XUHUA BIOLOGICAL TECH
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