Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for determining bio-available phosphorus in soil

It is a biologically effective and determination method, applied in the measurement of color/spectral properties, etc., which can solve the problems of cumbersome steps, insufficient contact between solid-state binding phases, and easy deviation from the theoretical design of DGT, and achieves simple operation, good absorption, and avoidance of morphological changes. Effect

Inactive Publication Date: 2014-12-10
BOHAI UNIV
View PDF1 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The DGT sampling device is mainly composed of a diffusion phase and a binding phase. The early-developed DGT sampling devices are all solid binding phases, which have two disadvantages: first, the contact between the solid binding phase and the solid diffusion phase is inevitably not close enough, and it is easy to deviate from the theoretical design of DGT; Second, the target substance enriched in the solid-state binding phase needs to be rinsed with acid, which is cumbersome and easily leads to measurement errors
Menzies, Mason and Ding et al. respectively studied the use of solid-state binding phase DGT sampling devices to measure the bioavailability of phosphorus in soil, but the use of liquid binding phase DGT sampling devices to measure the bioavailability of phosphorus in soil has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining bio-available phosphorus in soil
  • Method for determining bio-available phosphorus in soil
  • Method for determining bio-available phosphorus in soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Soil collection

[0032] Collect the corn rhizosphere soil to be tested (sampling depth is 0-20cm), air-dry naturally, remove plant residues, stones, etc. in the soil sample, pass through a 2mm nylon sieve after grinding, and mix well for later use;

[0033] 2) DGT sampling device installation

[0034] Dilute the purified ethylene didodecyl polyoxyethylene ether quaternary ammonium salt solution to 0.010mol / L, take the DGT sampling device whose material is polypropylene with a volume of 2mL, such as figure 1 As shown, this adopts DGT sampling device, comprises fixed plate 1, is provided with shell 2 with inner edge on fixed plate 1, is provided with support body 3 in shell 2, between the top of support body 3 and the inner edge of shell 2 The dialysis membrane 4 is mounted on the card, and an inverted tapered groove 301 is provided on the support body 3, and the groove 301 and the dialysis membrane 4 are surrounded to form a binding agent holding chamber 5; 2 mL of ...

Embodiment 2

[0047] 1) Soil collection

[0048] Collect the rhizosphere soil of the lettuce to be tested (sampling depth is 0-20cm), air-dry naturally, remove plant residues, stones, etc. in the soil sample, pass through a 2mm nylon sieve after grinding, and mix well for later use;

[0049] 2) DGT sampling device installation

[0050] The purified macromolecule cationic surfactant PCD solution is diluted to 0.050mol / L, and the material is taken as a DGT sampling device with a polytetrafluoroethylene volume of 2mL. The structure of the DGT sampling device is the same as in Example 1, and the DGT sampling device is packed into 2mL 0.050mol / L polymer cationic surfactant PCD solution, seal the DGT sampling device with a dialysis membrane with a molecular weight cut off of 7000;

[0051] 3) DGT sampling device placement

[0052] Weigh 500.00 g of air-dried soil passed through a 2 mm sieve, put it into a 1000 mL plastic container, add ultrapure water to make the water content in the soil to be...

Embodiment 3

[0063] 1) Soil collection

[0064] Collect the potato rhizosphere soil to be tested (sampling depth is 0-20cm), air-dry naturally, remove plant residues, stones, etc. in the soil sample, pass through a 2mm nylon sieve after grinding, and mix well for later use;

[0065] 2) DGT sampling device installation

[0066] The purified rosin-based quaternary ammonium salt solution was diluted to 0.050mol / L, and the material used was a DGT sampling device with a volume of 2mL of polypropylene. The structure of the DGT sampling device was the same as in Example 1, and 2mL of 0.050mol / For the rosin-based quaternary ammonium salt solution of L, the DGT sampling device is sealed by a dialysis membrane with a molecular weight cut-off of 7000;

[0067] 3) DGT sampling device placement

[0068] Weigh 300.00 g of air-dried soil passed through a 2 mm sieve, put it into a 600 mL plastic container, add ultrapure water to make the water content in the soil to be tested reach 60%, mix well, seal ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for determining bio-available phosphorus in soil. The method comprises the following steps: naturally airing collected plant rhizosphere soil, grinding the soil, sieving the soil with a nylon sieve with mesh size of 2mm, placing the soil into a plastic container, adding ultrapure water to enable the content of water in the soil to be tested to reach 60%, evenly mixing the soil with the water, sealing and then standing; adding ultrapure water again to enable the content of water in the soil to reach 80%, evenly mixing the soil with the water to enable the surface of the soil in the container to be smooth, sealing and then standing, thereby obtaining a soil test sample; diluting a purified binding agent solution, putting the solution into a DGT (Diffusive Gradient in Thin Film Technology) sampling device, and sealing the DGT sampling device by a dialysis membrane; slightly twisting the DGT sampling device with a hand so as to press the DGT sampling device into the surface of the soil test sample and enable the DGT sampling device to be in good contact with the soil, and standing; and taking out combined-phase soil, determining the cumulated amount of phosphorus in the combined-phase soil by an ammonium molybdate spectrophotometry process and calculating the concentration of available phosphorus in the soil. The method has the advantages that bio-available phosphorus in soil can be determined by virtue of a liquid combined phase DGT, the determined bio-available phosphorus is in significant correlation with the absorbed phosphorus in a plant body.

Description

technical field [0001] The invention relates to a method for measuring biologically available phosphorus in soil, in particular to a method for measuring biologically available phosphorus in soil by using liquid binding phase film gradient diffusion technology. Background technique [0002] Phosphorus is an essential nutrient element for crop growth, and the application of phosphorus fertilizer is an effective measure to increase soil phosphorus content and improve soil quality. Scientific fertilization is related to crop quality, agricultural production cost, soil nutrient balance, etc. In recent years, with the improvement of people's awareness of the importance of protecting resources and the environment, in order to curb the accelerated depletion of phosphate rock resources caused by continuous large-scale application of phosphate fertilizers, phosphorus in farmland soil is used as a non-point source of pollution to cause eutrophication of surface water and pollution of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/31
Inventor 陈宏励建荣张梦晗赵刚白凤翎
Owner BOHAI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com