Cryptomeria fortunei clone in-vitro rooting culture method

A technology for rooting culture and clones, applied in the field of plant rooting culture, can solve problems such as low seed germination rate, and achieve the effect of meeting the needs of industrial use and good practicability

Inactive Publication Date: 2014-12-24
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Carry out seedling raising by the mode of cutting and sowing, but there is low seed germination rate (mostly less than 10%)

Method used

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  • Cryptomeria fortunei clone in-vitro rooting culture method
  • Cryptomeria fortunei clone in-vitro rooting culture method
  • Cryptomeria fortunei clone in-vitro rooting culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Sterilization and bottle fixing of sprouts

[0025] Cut out the young and young shoots of the fine cedar cedar clone 011#, remove the excess leaves, soak in detergent diluted with deionized water for 30 minutes, and then rinse with running water for 1 hour. Under sterile conditions, soak in 70% alcohol for 90s, then treat with 0.1% mercuric chloride for 10 minutes, and then rinse with sterile water for 3-4 times, each time for 1-2 minutes. During the sterilization process of the medicinal solution, gently shake the triangular flask so that the sterilization solution fully contacts the explants. After rinsing, place it in a petri dish, and after the sterile filter paper absorbs the residual water, cut off the injured parts at both ends of the stem to reduce the poison of mercury liter. Then it was cut into 2-3cm long stem segments, inoculated on sterilized MS medium, and cultured for 2 weeks.

[0026] 2) Differentiation and elongation of buds

[0027] Put the tissu...

Embodiment 2

[0046] 1) Sterilization and bottle fixing of sprouts

[0047] Cut off young and young shoots of the fine cedar cedar clone 3# in the same year, remove excess leaves, soak in detergent diluted with deionized water for 30 minutes, and then rinse with running water for 1 hour. Under sterile conditions, soak in 70% alcohol for 90s, then treat with 0.1% mercuric chloride for 10 minutes, and then rinse with sterile water for 3-4 times, each time for 1-2 minutes. During the sterilization process of the medicinal solution, gently shake the triangular flask so that the sterilization solution fully contacts the explants. After rinsing, place it in a petri dish, and after the sterile filter paper absorbs the residual water, cut off the injured parts at both ends of the stem to reduce the poison of mercury liter. Then it was cut into 2-3cm long stem segments, inoculated on sterilized MS medium, and cultured for 2 weeks.

[0048] 2) Differentiation and elongation of buds

[0049] Put th...

Embodiment 3

[0066] 1) Sterilization and bottle fixing of sprouts

[0067] Cut out young and young shoots of the fine cedar cedar clone 70#, remove excess leaves, soak in detergent diluted with deionized water for 30 minutes, and then rinse with running water for 1 hour. Under sterile conditions, soak in 70% alcohol for 90s, then treat with 0.1% mercuric chloride for 10 minutes, and then rinse with sterile water for 3-4 times, each time for 1-2 minutes. During the sterilization process of the medicinal solution, gently shake the triangular flask so that the sterilization solution fully contacts the explants. After rinsing, place it in a petri dish, and after the sterile filter paper absorbs the residual water, cut off the injured parts at both ends of the stem to reduce the poison of mercury liter. Then it was cut into 2-3cm long stem segments, inoculated on sterilized MS medium, and cultured for 2 weeks.

[0068] 2) Differentiation and elongation of buds

[0069] Put the tissue materia...

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Abstract

The invention discloses a cryptomeria fortunei clone in-vitro rooting culture method. The method comprises steps as follows: 1), a tissue culture material is disinfected and cultured in a selected flask; 2), the tissue culture material is inoculated into a subculture multiplication medium for subculture multiplication, adventitious buds are induced and differentiated and then transferred into an MS (Murashige and Skoog) medium for elongation culture, the formula of the subculture multiplication medium adopts DCR, 1.0mg/L 6-BA, 0.05 mg/L of NAA and 3% of sugar; 3) when the adventitious buds elongate to 2-3 cm, the adventitious buds are transferred to a root induction medium for culture, a rooting test-tube plantlet is obtained, and the root induction medium adopts 1/2 DCR, 0.05-0.2mg/L of IBA, 0.05-0.2 mg/L of NAA and 2% of sugar; and 4), the rooting test-tube plantlet is transplanted. According to the method, coppice shoot is taken as a tissue culture material, and adventitious bud induction is performed through subculture multiplication; and when the adventitious buds elongate to 2-3 cm, 1/2 DCR is taken as a basic medium, a plant growth regulator is added for root induction, the average rooting percentage is 84% and can be up to 93.3% to the maximum, the average rooting number of each plantlet is 5.7, and the average survival rate of transplanting is larger than 86%.

Description

technical field [0001] The invention belongs to the technical field of rooting cultivation of plants, and in particular relates to a method for in vitro rooting cultivation of cedar clones. Background technique [0002] Cryptomeria (Cryptomeria. fortunei) is an evergreen tree, up to 40 meters high, with a diameter at breast height of more than 2 meters; the bark is reddish-brown, fibrous, split into long strips and falls off; the large branches are in whorls, flat or oblique; the branchlets are slender , often drooping, green, the leaves in the middle of the branches are longer, and often gradually become shorter towards the ends. Cedar natural forests are only found in Tianmu Mountain and Wuyi Mountain in southeast China, Honshu Island and Kyushu Island in Japan. Most plant taxonomists in China believe that there are two types of Cedar: Cedar (C. fortunei) and Japanese Cedar (C. japonica). Chinese cedar is a unique tree species in my country. It is produced in Tianmu Moun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 徐进祝晨辰施季森
Owner NANJING FORESTRY UNIV
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