Cell co-culture device

Abstract

The invention discloses a cell co-culture device. The cell co-culture device comprises a base, a culture medium agitating assembly, a culture dish filled with a culture medium and a plurality of movable cell culture chambers for accommodating cells, wherein the plurality of movable cell culture chambers are movably mounted in the culture dish, permeable membranes allowing non-cells to pass but refusing cells to pass are arranged on the movable cell culture chambers, the movable cell culture chambers are isolated from the culture dish by the permeable membranes, the culture medium agitating assembly is mounted on the base, and a rotating body on the culture medium agitating assembly extends into the culture dish, so that when the culture medium in the culture dish is stirred while the rotating body rotates, cell secretions in the movable cell culture chambers are transferred to corresponding target cells in the movable cell culture chambers. The cell co-culture device can co-culture more cells, avoid cross contamination among different cells, is favorable to observing and harvesting cells, can easily separate specific target cells in the co-culture process for the use of follow-up experiments and can stimulate the growth environment of in-vivo cells.

Description

technical field [0001] The invention relates to a cell co-cultivation device, belonging to the field of biotechnology, which is helpful to the research on the interaction and relationship between cells in the co-growth environment in the fields of cell biology and biomedicine. Background technique [0002] At present, the tumor microenvironment is a new concept proposed in recent years, which consists of tumor cells, extracellular matrix (ECM) and many stromal cells, including fibroblasts, immune and inflammatory cells, adipocytes, smooth muscle cells and Vascular endothelial cells, etc., are a complex comprehensive system. Studies have shown that tumors can induce changes in the local microenvironment to favor their own development. Various cells in the microenvironment interact with tumor cells, and produce a large number of cytokines, chemokines, matrix degrading enzymes, etc. around them, thus forming a large and complex network. More and more studies have confirmed th...

AI Technical Summary

Problems solved by technology

However, the existing co-cultivation technology has not been able to realize this function, and many patents are not involved in this work, such as patent application number: 201310093587.0, a double-layer cell co-culture device with adjustable gap; patent application number: 201210370447.9, cell culture device; patent application number: 201110242023.X, a variety of cell co-culture scaffolds

In addition, there are some deficiencies in the above-mentioned various patents, such as complex structure, cumbersome operation, etc., which need to be further improved.

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