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Bacterium for degrading acetochlor, and screening method and application thereof

A screening method, the technology of acetochlor, applied in the field of microorganisms, can solve the problems of soil pollution, acetochlor natural degradation time-consuming, aggravated soil pollution, etc., to achieve quick results, ideal screening results, and strong pertinence

Inactive Publication Date: 2014-12-24
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially with the large-scale use of acetochlor herbicide, the residual acetochlor in the soil gradually accumulates, causing serious soil pollution; in addition, the waste water and waste gas generated during the production of acetochlor pesticides are also discharged and lost. accumulated into the soil, further aggravating the pollution of the soil
However, the natural degradation of acetochlor takes a long time, which obviously cannot effectively solve the current situation of the gradual accumulation of acetochlor in soil

Method used

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  • Bacterium for degrading acetochlor, and screening method and application thereof
  • Bacterium for degrading acetochlor, and screening method and application thereof
  • Bacterium for degrading acetochlor, and screening method and application thereof

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Embodiment 1

[0038] The screening method for bacteria degrading acetochlor provided in this embodiment has the following steps:

[0039] Step 101: Place the mud sample to be tested in a basal salt liquid medium containing acetochlor, and culture it on a shaker to obtain the first mixed bacterial solution;

[0040] This step is the step of mixing the mud sample for testing with the basal salt liquid medium containing acetochlor, because in this medium, it has only included acetochlor as the nitrogen source (or carbon source) of microorganisms, so, During the cultivation process, all microorganisms that can decompose and utilize acetochlor can proliferate normally, while those that cannot utilize acetochlor for survival will die, and then the first mixed bacterial solution was preliminarily screened out.

[0041] Step 102: inoculating the first mixed bacterial solution into another basal salt liquid medium containing acetochlor, and performing subculture to obtain a second mixed bacterial so...

Embodiment 2

[0051] In this embodiment, the screening method for bacteria degrading acetochlor comprises the following steps:

[0052] S1: Put the mud sample to be tested in the basal salt liquid medium containing acetochlor, and cultivate it on a shaking table to obtain the first mixed bacterial liquid;

[0053] Specifically, in order to be able to find samples that can tolerate acetochlor and contain a large number of screened bacteria, and then obtain a higher screening effect; The sampling location, due to the high concentration of acetochlor at this location, the surviving microorganisms may utilize acetochlor to a greater extent, therefore, the wastewater drain from the acetochlor production plant makes the purpose of the sample Stronger, further contribute to the smooth progress of separation and screening.

[0054] In addition, by optimizing the ratio of the components of the basic salt liquid medium, the normal reproduction of microorganisms is ensured. Preferably, the basic salt...

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Abstract

The invention relates to the field of microbes, particularly a bacterium for degrading acetochlor, and a screening method and application thereof. The bacterium for degrading acetochlor is YCA-1 (Cupriavidus oxalaticus) which is collected by China General Microbiological Culture Collection Center; and the collection number is CGMCC No.9127. The YCA-1 can use the acetochlor as the nitrogen source and perform proliferation, and thus, can be used as a microbe for degrading acetochlor. The experiment proves that the YCA-1 can completely degrade the acetochlor in a basic salt culture medium with the acetochlor concentration of 50-150 mg / L under corresponding culture conditions within 5 days. In the concrete application process, the abundant propagated YCA-1 can be prepared into a degradation agent using the YCA-1 as the active component and used for restoring soil containing acetochlor insecticide.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterium for degrading acetochlor, a screening method and application thereof. Background technique [0002] Acetochlor, commonly known as Acetochlor in English, was first successfully developed by the American Monsanto Company in 1971, and its molecular formula is C 14 h 20 ClNO 2 , and its molecular weight is 269.5. The pure product of acetochlor is a colorless liquid, the original drug is a light yellow, brown or purple transparent liquid, and 50% acetochlor EC is a blue-purple oily substance, which is not easy to be photolyzed and volatile, with a boiling point greater than 200°C and a density of 1.11g / mL (30°C), decomposed under strong acid and strong base, soluble in various organic solvents such as methanol and acetone. [0003] Studies have shown that acetochlor can be used as a pre-emergence herbicide, and its mechanism of action is mainly to stop the growth of youn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02B09C1/10C02F3/34C12R1/01C02F101/38
Inventor 赵建庄李体文魏朝俊贾临芳吴昆明郑燕英梁丹
Owner BEIJING UNIV OF AGRI