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Method for removing nitrate nitrogen and total phosphorus in printing and dyeing wastewater by denitrifying poly-phosphorus accumulating organisms (B8)

A technology for denitrifying phosphorus-accumulating bacteria and printing and dyeing wastewater, applied in chemical instruments and methods, biochemical equipment and methods, methods based on microorganisms, etc., can solve problems such as non-performance of phosphorus removal function, autolysis death, etc.

Inactive Publication Date: 2014-12-24
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of obtaining denitrifying phosphorus accumulating bacteria in China, most of the treatment objects are phosphorus-rich medium or prepared simulated wastewater (which is also phosphorus-rich culture solution in essence), and the strains obtained in this way are added to printing and dyeing wastewater. Because of this, although a large number of phosphorus accumulating bacteria have been screened, few microbial preparations of phosphorus accumulating bacteria and dry powder agents of phosphorus accumulating bacteria are sold and promoted on the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] This embodiment treats 150mL of printing and dyeing wastewater, pH=7.0, TP=4.32mg / L, NO 3 - -N=35.81mg / L, COD=269.5mg / L, mainly for the nitrogen and phosphorus removal test of the anaerobic-aerobic process of the bacterial liquid.

[0025] The specific implementation steps are as follows: Centrifuge the B8 seed bacterial liquid for 3 times with sterile water at 4000r / min for 15 minutes, then add 10% of the bacteria into the printing and dyeing wastewater, and then perform anaerobic-aerobic process treatment. The specific operation of the anaerobic-aerobic process is as follows: put the water sample added with bacteria in a closed container and fill it with nitrogen, set the treatment temperature at 26°C, and incubate with rotor stirring for 4 hours. This is anaerobic treatment. Then put the water sample in an open container and culture it aerobically in a shaking table (26°C, 120r / min) for 21h, then take the water sample, then centrifuge the water sample (8000r / min, 15...

Embodiment 2

[0027] This embodiment treats 150mL of printing and dyeing wastewater, pH=7.0, TP=4.40mg / L, NO 3 - -N=36.15mg / L, COD=267.2mg / L, mainly for nitrogen and phosphorus removal test of anaerobic-aerobic process of bacterial liquid.

[0028] The specific implementation steps are as follows: Centrifuge the B8 seed bacterial liquid for 3 times with sterile water at 4000r / min for 15 minutes, then add 10% of the bacteria into the printing and dyeing wastewater, and then perform anaerobic-aerobic process treatment. The specific operation of the anaerobic-aerobic process is as follows: put the water sample added with bacteria in a closed container and fill it with nitrogen, set the treatment temperature at 30°C, and incubate with rotor stirring for 4 hours. This is anaerobic treatment. Then put the water sample in an open container and incubate aerobically in a shaking table (30°C, 120r / min) for 5h, then take the water sample, then centrifuge the water sample (8000r / min, 15min) to get the...

Embodiment 3

[0030] This example treats 150mL of printing and dyeing wastewater, pH=7.0, TP=4.38mg / L, NO 3 - -N=35.94mg / L, COD=274.4mg / L, mainly for the denitrification and dephosphorization test of the anaerobic-anoxic process of the bacterial liquid.

[0031] The specific implementation steps are as follows: Centrifuge the B8 seed bacterial liquid for 3 times with sterile water at 4000r / min for 15 minutes, then add 10% of the bacteria to the wastewater to be treated, and then perform anaerobic-anoxic process treatment. The specific operation of the anaerobic-anoxic process is as follows: place the water sample added with bacteria in a closed container and fill it with nitrogen, set the treatment temperature to 30°C, and incubate with rotor stirring for 4 hours. This is anaerobic treatment. Then put the water sample in an open container and shake it every 55min for 5min in an oscillating shaker (30°C, 60r / min) for 5min anoxic culture for 5h, then take the water sample, then centrifuge th...

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Abstract

The invention belongs to the technical field of sewage treatment, and particularly relates to a method for removing nitrate nitrogen and total phosphorus in printing and dyeing wastewater by denitrifying poly-phosphorus accumulating organisms (B8). The denitrifying poly-phosphorus accumulating organisms (B8) are preserved in the CGMCC (China general microbiological culture collection center) with the preservation number of CGMCC NO.9168. The denitrifying poly-phosphorus accumulating organisms are proved to be pseudomonas putida sp. 10% of a bacterium solution prepared by bacteria in percentage by mass is fed into printing and dyeing wastewater and treated by an anaerobic-aerobiotic process or an anaerobic-anoxic process for 9-25 hours at the temperature of 26-30 DEG C respectively, and nitrate nitrogen and total phosphorus in the printing and dyeing wastewater can be effectively removed.

Description

technical field [0001] The invention relates to a denitrifying phosphorus-accumulating bacterial strain (B8) for removing nitrate nitrogen (NO) in printing and dyeing wastewater. 3 - -N) and total phosphorus (TP) methods, belong to the technical field of sewage treatment. Background technique [0002] Denitrifying poly-phosphate accumulating organisms (DNPAOs) are a type of bacteria that use oxygen or nitrate nitrogen as electron acceptors to accumulate phosphorus. In an anaerobic environment, DNPAOs release polyphosphate (Poly- P) Obtain energy to absorb volatile fatty acids in water and store them as polyhydroxyalkanoate (PHA); consume PHA in an aerobic or anoxic environment, and use oxygen or nitrate nitrogen as electron acceptors for excess absorption Phosphorus in water, and re-synthesis of Poly-P in the strain. Using denitrifying phosphorus-accumulating bacteria to carry out biological nitrogen and phosphorus removal not only saves the cost of adding chemical agents...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/40
Inventor 张文艺陈晶邓文姚立荣程寒飞胡林潮
Owner CHANGZHOU UNIV
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