Novel application of radiation-resistant peptide proteins
A technology of radioresistant peptides and proteins, which is applied in the field of new applications of radioresistant peptides and proteins, and can solve problems such as unreported effects
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Embodiment 1
[0051] Embodiment 1, the preparation of radioresistant peptide protein (FKT)
[0052] The artificially synthesized radioantipeptide protein (FKT) encoding gene sequence 1, the amino acid sequence of the encoded protein radioantipeptide (FKT) is sequence 2 in the sequence list.
[0053] The FKT coding gene shown in Sequence 1 was inserted between the SmalI and SalI sites of the pBV220 prokaryotic expression vector to obtain a recombinant vector, which was named pBV220-FKT.
[0054] Transform the radioresistant peptide expression plasmid pBV220-FKT into BL21 Escherichia coli to establish expression engineering bacteria, and cultivate them at 30°C until the cell density is OD 600 =0.6 or so, raise the temperature to 42°C to induce the expression of the radioresistant peptide gene, and after 4 hours of induced expression, the bacteria are collected by centrifugation, crushed by ultrasonication, and the precipitated part retained by high-speed centrifugation is the crude inclusion ...
Embodiment 2
[0057] Example 2. Application of radioantipeptide in inhibiting liver injury
[0058] 1. Radioantipeptide reduces the mortality of mice with liver injury caused by Con A
[0059] The mouse model of acute liver injury was established: 8-week-old C57BL / 6 male mice (body weight 18-22 g) were bred in a barrier environment, and the light alternating time was 12 hours; concanavalin A (hereinafter referred to as Con A, C7275, Sigma company) induced acute liver injury model, Con A lethal dose is 25mg / kg body weight in this model, Con A is diluted to 25mg / kg with the NaCl aqueous solution (physiological saline) of concentration of 0.9% to obtain Con A solution with sterile injection , injected rapidly through the tail vein of mice with an injection volume of 200 μl.
[0060] The specific experiment is as follows:
[0061] Divide C57BL / 6 male mice into two groups of 12 mice each:
[0062] Experimental group (injection of radioantipeptide protein): 24 hours, 12 hours, 6 hours and 0.5 ho...
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