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Novel application of radiation-resistant peptide proteins

A technology of radioresistant peptides and proteins, which is applied in the field of new applications of radioresistant peptides and proteins, and can solve problems such as unreported effects

Inactive Publication Date: 2015-01-07
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the only ligand of TLR5, the role of flagellin or its derivative radioantipeptide protein in the protection of liver injury has not been reported yet

Method used

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  • Novel application of radiation-resistant peptide proteins
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  • Novel application of radiation-resistant peptide proteins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, the preparation of radioresistant peptide protein (FKT)

[0052] The artificially synthesized radioantipeptide protein (FKT) encoding gene sequence 1, the amino acid sequence of the encoded protein radioantipeptide (FKT) is sequence 2 in the sequence list.

[0053] The FKT coding gene shown in Sequence 1 was inserted between the SmalI and SalI sites of the pBV220 prokaryotic expression vector to obtain a recombinant vector, which was named pBV220-FKT.

[0054] Transform the radioresistant peptide expression plasmid pBV220-FKT into BL21 Escherichia coli to establish expression engineering bacteria, and cultivate them at 30°C until the cell density is OD 600 =0.6 or so, raise the temperature to 42°C to induce the expression of the radioresistant peptide gene, and after 4 hours of induced expression, the bacteria are collected by centrifugation, crushed by ultrasonication, and the precipitated part retained by high-speed centrifugation is the crude inclusion ...

Embodiment 2

[0057] Example 2. Application of radioantipeptide in inhibiting liver injury

[0058] 1. Radioantipeptide reduces the mortality of mice with liver injury caused by Con A

[0059] The mouse model of acute liver injury was established: 8-week-old C57BL / 6 male mice (body weight 18-22 g) were bred in a barrier environment, and the light alternating time was 12 hours; concanavalin A (hereinafter referred to as Con A, C7275, Sigma company) induced acute liver injury model, Con A lethal dose is 25mg / kg body weight in this model, Con A is diluted to 25mg / kg with the NaCl aqueous solution (physiological saline) of concentration of 0.9% to obtain Con A solution with sterile injection , injected rapidly through the tail vein of mice with an injection volume of 200 μl.

[0060] The specific experiment is as follows:

[0061] Divide C57BL / 6 male mice into two groups of 12 mice each:

[0062] Experimental group (injection of radioantipeptide protein): 24 hours, 12 hours, 6 hours and 0.5 ho...

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Abstract

The invention discloses novel application of radiation-resistant peptide proteins and provides application of radiation-resistant peptide proteins in preparation of products for inhibiting liver injury diseases. The amino acid sequence of the radiation-resistant peptide proteins refers to a sequence 2 in a sequence table. The experiment proves that the radiation-resistant peptide proteins can inhibit the liver injury and can be used for preparing medicines with the effect of inhibiting the liver injury.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the new application of radioresistant peptide protein. Background technique [0002] Toll-like receptors (Toll-like receptors, TLRs) were discovered by Lemaitre et al. in the study of Drosophila in 1996. Current research shows that 11 members of the TLRs family have been found in the human body, mainly expressed in immune cells. The Toll-like receptors (TLRs) family is a class of pattern recognition receptors that play an important role in the body's innate and acquired immune responses. They activate the NF-κB signaling pathway through specific recognition of pathogen-related molecular patterns such as bacteria and viruses. Induces inflammatory response, antiviral response and differentiation and maturation of immune effector cells, and thus resists the invasion of pathogenic microorganisms. [0003] Studies have shown that a variety of TLR receptors are expressed in liver cells an...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P1/16
Inventor 杨晓明葛常辉詹逸群王磊
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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