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Method for extracting and separating L-tryptophan from nitraria tangutorum bobr seeds

A technology of Nitraria japonica seeds and tryptophan, which is applied in the field of separation and extraction of L-tryptophan, and achieves the effects of simple operation, high safety, and environmentally friendly process

Active Publication Date: 2015-01-07
浙江梓迪科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Also do not see the method that adopts macroporous adsorption resin to extract and separate L-tryptophan from Nitraria seeds

Method used

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  • Method for extracting and separating L-tryptophan from nitraria tangutorum bobr seeds
  • Method for extracting and separating L-tryptophan from nitraria tangutorum bobr seeds
  • Method for extracting and separating L-tryptophan from nitraria tangutorum bobr seeds

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Embodiment 1

[0025] Embodiment 1: L-tryptophan separation and extraction method

[0026] (1) After taking 5 kg of Nitraria seeds and pulverizing them, add 75% ethanol and carry out thermal extraction in a decoction machine at an extraction temperature of 60° C. and an extraction time of 2 hours, and extract three times altogether. The first feed-to-liquid ratio is 1:15, the second feed-to-liquid ratio is 1:12, and the third feed-to-liquid ratio is 1:10. The extracts were combined and concentrated under reduced pressure to 250 mL, then extracted 5 times with petroleum ether, and the extraction ratio of petroleum ether was 250 mL of crude extract and 750 mL of petroleum ether.

[0027] (2) Specifications of macroporous resin column: diameter 5 cm, column height 1.2 m

[0028] Column loading: Take 1.5kg D101 macroporous adsorption resin, put it into the chromatographic column, perform desorption treatment after three days of adsorption, flush the column with water and collect samples for eac...

Embodiment 2

[0036] Embodiment 2: L-tryptophan separation and extraction method

[0037] (1) Take 5 kg of Nitraria seeds, grind them, add 75% ethanol, and heat extract in a decoction machine. The extraction temperature is 60° C., and the extraction time is 3 hours. A total of three extractions are performed. The first feed-to-liquid ratio is 1:12, the second feed-to-liquid ratio is 1:10, and the third feed-to-liquid ratio is 1:8. The liquid was taken and concentrated under reduced pressure to 200mL, and then extracted 5 times with petroleum ether, and the extraction ratio of petroleum ether was 200mL of crude extract and 700mL of petroleum ether.

[0038] (2) Specifications of macroporous resin column: diameter 5 cm, column height 1.2 m

[0039] Column loading: Take 1.5kg D101 macroporous adsorption resin, put it into the chromatographic column, perform desorption treatment after three days of adsorption, flush the column with water and collect samples for each column volume (column volum...

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Abstract

The invention discloses a method for extracting and separating L-tryptophan from nitraria tangutorum bobr seeds. The method comprises operation steps as follows: (1), the nitraria tangutorum bobr seeds are taken and extracted by taking ethanol or hydrous ethanol as a solvent, extracting solutions are combined, ethanol is recovered, concentration is performed, petroleum ether is adopted for extraction, and an aqueous layer is collected; (2), the extraction liquor is taken to pass macroporous adsorption resin columns, water is adopted for elution, water eluent is collected, and a crude product of L-tryptophan is obtained after concentration and drying; and (3), the crude product of L-tryptophan is taken and purified to obtain a pure product of L-tryptophan. According to the research of the invention, the nitraria tangutorum bobr seeds are taken as raw materials, macroporous resin is adopted for washing, and then high-purity crude and pure products of L-tryptophan are obtained through separation and extraction. The eluting part is totally different from that in the prior art, the eluting technology only requires a washing liquid, the operation is simpler and more convenient, the cost is lower, and meanwhile, a new choice is provided for development and utilization of the nitraria tangutorum bobr seeds.

Description

technical field [0001] The invention relates to a method for separating and extracting L-tryptophan. Background technique [0002] Nitraria Tangutorum Bobr (Nitraria Tangutorum Bobr) is a plant of the genus Nitraria (Zygophyllaceae) in the family Zygophyllaceae. It is a medicinal and edible plant that mainly grows in desert or semi-desert areas. Nitraria tangutica has the characteristics of drought resistance, salt and alkali resistance, and sand burial resistance, making it a rare shrub species for windbreak and sand fixation, water and soil conservation, and soil improvement. Tangut Nitraria is called Kami in the "Dictionary of Traditional Chinese Medicine". It is harvested when the fruit is ripe, dried and used as medicine, which has the effect of invigorating the spleen and stomach. Nourishes and strengthens, regulates menstruation and activates blood circulation, mainly treats diseases such as emaciation, deficiency of qi and blood, disharmony between spleen and stomac...

Claims

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Application Information

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IPC IPC(8): C07D209/20
CPCC07B2200/07C07D209/20
Inventor 索有瑞胡娜周武
Owner 浙江梓迪科技有限公司
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