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Preparation method of polyhydroxy sterol monomeric compound and application

A technology for polyhydroxy sterols and compounds, which is applied in the application field of polyhydroxy sterol monomer compounds, can solve problems such as the anti-artemia activity of chemical components that have not yet been seen, and achieve the effects of simple preparation method, high efficiency and shortening cycle.

Active Publication Date: 2015-01-07
广西海洋研究所有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the anti-artemia activity of its chemical constituents

Method used

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  • Preparation method of polyhydroxy sterol monomeric compound and application
  • Preparation method of polyhydroxy sterol monomeric compound and application
  • Preparation method of polyhydroxy sterol monomeric compound and application

Examples

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Embodiment 1

[0013] Preparation:

[0014] (1) Take fresh sea squirts with a wet weight of 1.5 kg, homogenize them in a tissue masher, extract them four times with 95% ethanol ultrasonic waves, each time for 30 minutes, combine the extracts, and concentrate the extracts in a rotary evaporator to 35 When the thermal relative density at ℃ is 1.1-1.4, 1.5g of the extract is obtained. Disperse the extract with 1500g of water. After dispersion, extract with an equal amount of ethyl acetate. When the extract is concentrated under reduced pressure to 35℃, the thermal relative density is 1.1- At 1.4, 700.0 mg of ethyl acetate phase extract was obtained;

[0015] (2) Dissolve the ethyl acetate phase extract fully with methanol, use Sephadax LH-20 gel column chromatography, methanol elution, then use RP-18 reverse phase silica gel column chromatography, methanol / water concentration is respectively 30% , 40%, 50%, 60%, 70%, 80%, 90%, 100% for gradient elution, and finally preparative high performanc...

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Abstract

The invention discloses a preparation method of a polyhydroxy sterol monomeric compound and an application. The preparation method comprises the following steps: triturating and uniformly homogenizing fresh styela plicata, carrying out ultrasonic extraction by 95% ethanol, decompressing and concentrating to obtain an extract, dispersing the extract by using water, extracting by virtue of ethyl acetate, and carrying out concentration under reduced pressure on the extraction liquid to obtain an ethyl acetate liquid extract; fully dissolving the ethyl acetate liquid extract by methanol, carrying out SephadaxLH-20 gel column chromatography and RP-18 reverse silica-gel column chromatography; and finally, preparing by using efficient high performance liquid chromatography to respectively obtain a compound 1, dendronesterol A and a compound 2 (20S, 22E, 24R)-ergosta-7, 22-dien-3beta, 5alpha, 6beta-triol, wherein the artemia resistant activity of the compounds 1 and 2 is detected, and the fatality rates to artemia are respectively 87.4% and 82.9% when the concentration is 50 microgramme / mL and rise along with the concentration, showing concentration dependence. The compound can be applied to preparing artemia-resisting drugs. The compound which can be applied to preparing artemia-resisting drugs has easily available raw materials and is wide in prospect.

Description

technical field [0001] The present invention relates to a preparation method of a polyhydroxy sterol monomer compound, and also relates to the application of a polyhydroxy sterol monomer compound. The polyhydroxy sterol monomer compound refers to a monomer component extracted and separated from Ascidian ascidian, Belongs to the field of marine natural product chemistry. Background technique [0002] Since the 1980s, some natural product researchers in the world have discovered compounds with anti-tumor and anti-viral activities from sea squirts, and many of them have entered clinical research, making sea squirts a significant pharmacological agent for human beings in addition to sponges and corals. Important marine living resource of active substances. There are many studies on the nutritional components of sea squirts at home and abroad, such as amino acids, mineral elements, fatty acids and polysaccharides, etc., which are of considerable benefit to the human body and are...

Claims

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Application Information

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IPC IPC(8): C07J9/00A01N45/00A01P7/00
CPCA01N45/00C07J9/00A01N2300/00
Inventor 孙雪萍徐艳黄国强杨家林刘海娟
Owner 广西海洋研究所有限责任公司
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