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Large-scale microalgae cultivation method

A microalgae culture and large-scale technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost of construction and use of photobioreactors, achieve high light energy utilization efficiency, reduce production Cost, save the effect of cultivating floor space

Inactive Publication Date: 2015-01-07
李健
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Problems solved by technology

The photobioreactor can provide a closed culture environment and controllable culture conditions. In theory, it can relatively well cultivate most microalgae species. It has been the focus of research and development in the industry for many years. However, there are still construction and The problem of high cost of use is only applicable to the production of high value-added microalgae products, and its application in the microalgae industry has certain limitations. A complete set of mature process routes has not yet been formed for large-scale microalgae cultivation methods

Method used

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Embodiment 1

[0058] The present invention, a kind of method for large-scale microalgae cultivation, it specifically comprises the following steps:

[0059] Step 1, laboratory expansion of Haematococcus pluvialis

[0060] Firstly, the present invention preferably selects the BBM medium formula suitable for the growth of microalgae to configure the microalgae liquid medium, and adds 1% agarose gel to the liquid culture to make the solid medium;

[0061] Secondly, preserve and cultivate microalgae in the incubator, the culture temperature is 20°C, and the light intensity is about 30uE / m 2 / s;

[0062] Then, transfer the microalgae from the solid medium to a 200ml culture bottle containing 20mL of medium, and culture them in a rocking boat incubator for about 14-21 days at a culture temperature of 20°C and a light intensity of about 30uE / m 2 / s, shaker about 40rpm;

[0063] Finally, select the culture bottle whose color of the algae fluid turns green, observe the appearance of the algae flu...

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Abstract

The invention discloses a large-scale microalgae cultivation method, and belongs to the technical fields of microalgae cultivation and production. The method comprises the following steps: firstly, carrying out microalgae seed holding and enlarging cultivation in a laboratory, and then carrying out large-scale microalgae species solution cultivation in an outdoor light bioreactor; inoculating microalgae species solutions cultivated by using the light bioreactor into an outdoor runway culture pond, and carrying out large-scale microalgae cultivation in the runway culture pond; and finally obtaining microalgae biomass from the runway culture pond, and producing the microalgae biomass-based product. According to the method, through the combination of the outdoor light bioreactor and the runway culture pond, large-scale microalgae cultivation can be carried out; compared with the microalgae cultivated by a pure runway culture pond or a pure light bioreactor, the production cost is significantly reduced; and a whole set of complete process route is developed.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation and production, and in particular relates to a method for large-scale microalgae cultivation that can be used to produce bioenergy, biomaterials, food, feed, health care products and medicines. Background technique [0002] An important reason why microalgae, as a valuable biological resource, has not been fully utilized is that there is no economical production system. At present, the methods used for microalgae cultivation include raceway culture method, photobioreactor culture method, etc. However, the raceway culture tank required for the raceway culture method can only cultivate a few species grown in selective media. Algae species, such as microalgae species such as spirulina and salina; while the closed photobioreactor system used in the photobioreactor culture method can cultivate most microalgae species, but the construction cost and operation cost are high. This situation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCC12N1/12C12P23/00
Inventor 李健郭素霞韩睿明张兆雷斯波柔斯.阿哥图斯
Owner 李健
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