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Extraction method of cephalosporin C

A cephalosporin and extraction method technology, applied in the field of antibiotics, can solve the problems of unstable cephalosporin C, poor quality of 7-ACA, poor quality of desorbed liquid, etc. The effect of reducing losses

Active Publication Date: 2015-01-14
SHANGHAI KAIXIN ISOLATION TECH CO LTD
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Problems solved by technology

However, due to the instability of cephalosporin C
Loss of cephalosporin C during pretreatment, especially acid addition
Typically the process loses 4-10% of the yield
Moreover, even after pretreatment and conventional ultrafiltration, although the quality of the filtrate is significantly improved compared with plate and frame or other traditional filtration methods, there is still a large amount of protein in the filtrate. For the subsequent resin process, resin pollution is still caused, and the quality of the desorption liquid is not good. Well, the quality of 7-ACA obtained directly by enzymatic hydrolysis is poor

Method used

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Experimental program
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Embodiment Construction

[0029] The temperature of the cephalosporin fermented liquid of the cephalosporin that has been fermented is 25-28 DEG C, the pH is neutral, and the suspended solids are 40-60%. The fermentation unit is 20000-40000. The fermented liquid is rapidly cooled to below 10°C through a heat exchanger, and then enters the primary ultrafiltration system.

[0030] After the treatment of the ultrafiltration membrane with a molecular weight cut-off of 10000-50000 in the primary ultrafiltration system, the insoluble substances and most of the dissolved proteins, polypeptides, polysaccharides, dextrins and various colloids are removed, and the filtrate is clear and transparent. The clear and transparent filtrate directly enters the secondary ultrafiltration system. The secondary ultrafiltration system has a molecular weight cut-off of 800-10000, which further removes impurities such as small molecular proteins, small molecular peptides, polysaccharides, and pigments dissolved in the primary...

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Abstract

An extraction method of cephalosporin C mainly comprises the steps of primary ultrafiltration, secondary ultrafiltration, macroporous resin adsorption, weakly alkaline resin adsorption and nanofiltration concentration. An ultrafiltration combination technology is adopted to directly filter a fermentation liquid, and the pH value of the obtained clarified filtrate is adjusted by sulfuric acid. The technology effectively reduces the loss of cephalosporin C in the acid adjustment process. The filtrate obtained through the ultrafiltration combination technology has the advantages of good light transmittance, and thorough removal of proteins and other impurities, and the method has the advantages of product quality improvement, increase of the extraction yield of a resin workshop section, and prolongation of the recovery cycle and the service life of resin, and obtained 7-ACA (7-aminocephalosporanic acid) has good quality and good stability.

Description

technical field [0001] The invention relates to antibiotics, in particular to a method for extracting cephalosporin C. Background technique [0002] Cephalosporin C removes the side chain to obtain 7-aminocephalosporanic acid (7-ACA), which is an important raw material for the synthesis of cephalosporin series antibiotics and an important pharmaceutical intermediate. Cephalosporin antibiotics have become the largest class of antibiotics in the market for anti-infective drugs because of their broad antibacterial spectrum, strong antibacterial activity, and low adverse reactions and side effects. [0003] In the production process of 7-ACA, cephalosporin C is first produced, and then the side chain is removed from cephalosporin C to obtain 7-ACA. [0004] Cephalosporin C is isolated from the fermentation broth of Cephalosporium crown. It is obtained through biological fermentation with cornstarch, corn steep liquor, soybean oil, etc. as raw materials. The usual fermentation...

Claims

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Application Information

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IPC IPC(8): C12P35/06C12P35/02C07D501/28C07D501/12
Inventor 田灿彬申雅维曹厚康
Owner SHANGHAI KAIXIN ISOLATION TECH CO LTD
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