Indoor fast spider mite population propagation method

A kind of population and rapid technology, applied in animal husbandry, etc., can solve the problems of small number of leaves, short feeding time, affecting the normal growth and reproduction of spider mite populations, etc., to achieve simple breeding technology, fast growth speed, and guaranteed succession maintenance and multiplication effects

Inactive Publication Date: 2015-01-21
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, there are the following obvious disadvantages in the reproduction of spider mite populations in kidney beans: first, the leaves of kidney beans are relatively thin, and they quickly turn white and dry after spider mites infestation, requiring frequent replacement of new leaves. The sponge-water isolation table method is the longest breeding It can only last for 5-7 days and needs to be replaced once, so it takes a lot of work to raise; second, other small pests such as whitefly, leafminer, and thrips often occur on the kidney beans, and they interact with spider mites for ecological niche and nutrition. Third, the number of leaves of living bean plants is small, and when a large number of spider mites multiply, the time for the spider mites to feed is relatively short, and the plants need to be replaced frequently

Method used

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  • Indoor fast spider mite population propagation method
  • Indoor fast spider mite population propagation method
  • Indoor fast spider mite population propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Indoor rapid multiplication of spider mite populations

[0016] 1. Test spider mite

[0017] The tested spider mite was Tetranychus urticae, which was subcultured indoors in an artificial climate chamber by the sponge-water isolation table method, and the host leaves were insect-free bean seedlings (the variety is "Bifeng"). The feeding condition is 26±1°C, the relative humidity is about 80%, and the photoperiod is 16h:8h (L:D).

[0018] 2. Spontaneous process of peanut seedlings

[0019] Indoor cultivation of insect-free peanut seedlings, the specific process is: select new peanut species with full grains, uniform size, and no damage, first wash the seeds with clean water, and remove the seeds, seed coats and impurities floating on the water surface. Soak overnight, the next day, 2 of the soaked seeds are placed in a group of nutrient pots with water-drenched vermiculite soil (when there are many peanut seedlings, the soaked seeds can also be evenly sprinkl...

Embodiment 2

[0021] Embodiment 2 Peanut and kidney bean are used as the contrast of host culture Tetranychus urticae

[0022] 1. Lifespan and egg production of Tetranychus urticae on the leaves of beans and peanuts

[0023] Peanut leaves and bean leaves are respectively punched into leaf butterflies of the same size (2 cm in diameter) with a puncher. The leaf butterfly was placed backside up on top of damp filter paper in a Petri dish (3.5 cm in diameter). Each leaf butterfly was connected with 10 newly hatched female adult mites within 24 hours, covered with Parafilm film, and repeated 4 times. After 24 hours, the female adult mites were removed, and 10 eggs were left on each leaf butterfly. Observe the growth and development of spider mites every day, record the development time from eggs to adults, and pick out each adult mite separately on a single leaf butterfly after it develops into adults. The lifespan of male and female adults and the amount of eggs laid by female adults were r...

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Abstract

The invention relates to the field of plant protection, in particular to an indoor fast spider mite population propagation method. The method includes cultivating peanut seedlings without insects and mites indoors; using the sprouted peanuts after 15 to 20 days after sprouting, placing adult spider mites to leaves of peanuts, placing the peanut seedlings with the mites to an artificial climate box with the same spider mite feeding condition, and allowing the spider mites to spawn, incubate and establish populations. According to the method, the egg volume on the peanut leaves is increased significantly. The method has the advantages that the host plant can complete the life history successfully, the speed for population increasing is high, the host plant is simple to cultivate, spider mite population subculture maintenance and propagation are guaranteed, and the host plant for propagating the spider mite population is selected according to the natural host the spider mites prefer.

Description

technical field [0001] The invention relates to the field of plant protection, in particular to a method for quickly multiplying spider mite populations indoors. Background technique [0002] Subculture and mass breeding of spider mite populations are the premise and basis for basic research and population management of pest mites. At present, the commonly used method for indoor breeding of spider mite populations is to adopt the sponge-water isolation table method to carry out indoor small population breeding, and when a large number of populations are needed to multiply populations, living kidney bean plants are often used (Zhao Weidong et al., Acta Entomology, 2003, 46 (6 ): 788-792; Van Nieuwenhuyse et al., Pest Management Science, 2009, 65:404-412). However, there are the following obvious disadvantages in the reproduction of spider mite populations in kidney beans: first, the leaves of kidney beans are relatively thin, and they quickly turn white and dry after spider ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 王少丽王玲张友军吴青君谢文徐宝云
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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