Method for preparing antibody target modified Alzheimer disease targeted drug delivery compound and application of antibody target modified Alzheimer disease targeted drug delivery compound
A technology of Alzheimer's disease compound, which is applied in the field of preparation of Alzheimer's disease targeted drug delivery compound, to achieve the effect of improving bioavailability and long half-life
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Embodiment 1
[0030] Take hydrogenated soybean lecithin (PC), cholesterol (Cholesterol) and DSPE-mPEG2000 in a 1.5 ml EP tube, heat and dissolve in 0.3 ml ethanol solution (65°C), draw it into a 1 ml syringe; take 3 ml Heat 250 mM ammonium sulfate solution in a glass bottle at 65°C, add ginsenoside under magnetic stirring to dissolve completely; take out the syringe, slowly inject the oil phase in the syringe into the water phase, and stir magnetically for 10-15 minutes to form a ginsenoside-coated Liposomes: react overnight with thiolated antibodies and ginsenoside-coated liposomes at room temperature with stirring, the maleic anhydride active groups on the surface of the carrier react with thiolated antibodies, and finally use a 50K dialysis membrane to remove the free antibodies The antibody-targeted drug nanocarrier is prepared by dialysis.
Embodiment 2
[0032] Preparation of the test sample: Dilute the dialyzed targeted drug nanocarrier 10 times with MilliQ-water; take 0.5mL of the sample to be tested, add it to the sample pool, and confirm that there are no bubbles in the measurement container. Each sample was measured 3 times. The measurement conditions are: 170° scattering angle, 25°C.
Embodiment 3
[0034] Highly differentiated PC12 cells were seeded in a 96-well plate at a density of 5000 cells / well and incubated at 37°C with 5% CO2. Set up 0, 10, 15, 20, 25, 30 μM amyloid-treated experimental groups and a blank control, and set 3 replicate wells for each group. After 24 hours of amyloid treatment, add CCK-8 reagent, the volume ratio of medium:CCK-8 reagent is 10:1 (CCK-8 reagent 10 μL), shake well on the shaker, and incubate the cells in the incubator for 1.5 h. A microplate reader (TECAN Model Sa?re2) was used to measure the OD value of each well at a wavelength of 450 nm.
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