Method for quickly inducing, sub-culturing and regenerating spirodela calli

A technology of callus induction and callus, applied in botany equipment and methods, plant regeneration, horticultural methods, etc., can solve problems such as inapplicable Ziping regeneration system

Active Publication Date: 2015-01-28
HUBEI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the patent CN 102487827 B discloses a duckweed regeneration system, experiments show that this technology is not suitable f

Method used

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  • Method for quickly inducing, sub-culturing and regenerating spirodela calli
  • Method for quickly inducing, sub-culturing and regenerating spirodela calli
  • Method for quickly inducing, sub-culturing and regenerating spirodela calli

Examples

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Embodiment 1

[0024] Embodiment 1: the method for Ziping callus induction, subculture and regeneration provided by the present invention

[0025] 1). Take the Vigorously growing Zipingia plant, and cross-cut its leaf base, place the treated explants in the Zipingia induction medium, under light 16h / darkness 8h, light temperature 26°C / dark temperature 24 ℃, light intensity 70-80μmol / m 2 Under the culture condition of s, the explants began to expand after 10 days, and the callus began to form after two weeks ( figure 1 ), the green part was selected and moved to Ziping subculture medium for subculture, and after 4 weeks, a well-growing callus ( figure 2 ), the callus was subcultured once every two weeks;

[0026] 2). The Ziping callus is moved to the regeneration medium, and the culture conditions are the same as step 1). After 15 days, the Ziping callus regeneration rate reaches 90% ( image 3 ), after 20 days, the regeneration rate was up to 100%, and the regeneration plant was moved in...

Embodiment 2

[0035] Example 2: Comparative experiment of using the method of the present invention and the method of treating duckweed in the patent CN 102487827 B to process purple duckweed

[0036] A). Utilize the inventive method to process purple duckweed:

[0037] 1). Take a vigorously growing plant of Zipingia chinensis, cut its leaf base transversely, and place the treated explants in the culture medium of Zipingiasis callus induction. Under light 16h / darkness 8h, light temperature 26°C / dark temperature 24°C, light intensity 70-80μmol / m 2 s, the explants began to expand after 10-15 days, and the callus began to form after two weeks ( figure 1 ), select the green part and move it on the Ziping subculture medium for subculture, and after 4 weeks, a well-growing callus ( figure 2 ): the callus was subcultured every two weeks;

[0038] 2). The Ziping callus was moved to the regeneration medium, and the culture conditions were the same as in step 1). After 15 days, the Ziping callus ...

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Abstract

The invention provides a method for quickly inducing, sub-culturing and efficiently regenerating spirodela calli. A rapid spirodela regeneration system is firstly created by taking spirodela of spirodela schleid of lemna minor as a material and by inducing and sub-culturing the spirodela calli, and regenerating the spirodela calli. According to the method, the formation of spirodela regeneration plants can be efficiently induced by using a hormone-free formula; the regeneration time is shortened to two weeks; both the regeneration rate of the regeneration plants and the regeneration rate of spirodela roots reach 100 percent. The efficient spirodela rapid regeneration system established by the method can be applied to related research and production work very well and also provides an important technical platform and support for the research of transgenosis and the like.

Description

technical field [0001] The invention relates to a method for regeneration of plant callus, in particular to a method for inducing fast and efficient regeneration of the aquatic floating plant Zima genus Spirodela of the family Lemnaceae with a hormone-free formula. Background technique [0002] Spirodela polyrrhiza (Spirodela polyrrhiza, also known as multiple roots) is an aquatic floating plant of the genus Spirodela (Spirodela) in the family Duckweed (1emnaceae). Aquatic floating plants. The amounts of macroelements, microelements, organic matter and phytohormones required for optimal growth of plants of different genera of Duckweed are very inconsistent, resulting in differences in the induction, differentiation and phylloid formation conditions of their callus, and from evolution In terms of relationship, Zima is the most primitive type in Duckweed, and its genome is smaller than that of other Duckweed plants. The growth conditions required for induction, differentiati...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王友如
Owner HUBEI NORMAL UNIV
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