A reagent erythrocyte preserving fluid and a preparing method thereof

Abstract

The invention relates to a reagent erythrocyte preserving fluid and a preparing method thereof, and belongs to the field of medical examination. The preserving fluid comprises distilled water and sodium chloride. The preserving fluid and the method are characterized in that: the preserving fluid also comprises sodium citrate, ammonium chloride, disodium hydrogen phosphate, glucose, mannitol, adenine, chloramphenicol, sodium azide and amino acids, and the weight ratio of the distilled water, the sodium citrate, the ammonium chloride, the disodium hydrogen phosphate, the glucose, the mannitol, the adenine, the chloramphenicol, the sodium azide, the amino acids and the sodium chloride is 100 : 2-3 : 0.5-1 : 0.1-0.2 : 4-5 : 1-2 : 0.1-0.2 : 0.15-0.2 : 0.001-0.0025 : 1-2 : 2-3. By using the preserving agent for reagent erythrocyte preservation, the storage lifetime of the reagent erythrocytes is largely prolonged and can reach 90 days, thus largely facilitating fully-automatic micro-plate scan detection of reverse ABO typing for a large number of blood donors in a blood station, and carrying out of hemolytic disease of the newborn and difficult blood matching which are caused by incompatibility of blood group between the mother and fetus, and other works, and laying good foundations for serology examination and research on erythrocyte blood types.

Description

technical field [0001] The invention relates to a reagent erythrocyte preservation solution and a preparation method thereof, belonging to the field of medical examination. Background technique [0002] In the existing ABO anti-typing full-automatic scanning and other blood group serological work, fresh reagent red blood cells are preserved with 5% normal saline, and the preservation period is 3 days. It is inconvenient to carry out work such as reverse typing of ABO blood type of a large number of blood donors in the blood station, hemolytic disease of the newborn caused by incompatibility of blood type of mother and baby, and difficult blood matching. Contents of the invention [0003] The purpose of the present invention is to find out a kind of new reagent erythrocyte preservation liquid and preparation method, and reagent erythrocyte is preserved in reagent erythrocyte preservation liquid, can prolong the reagent erythrocyte preservation period greatly, reaches 90 day...

AI Technical Summary

Problems solved by technology

It is inconvenient for the development of ABO blood type reverse stereotyping of large quantities of blood donors at the blood sta