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A kind of animal urine dry powder for quality control of clenbuterol detection and its preparation method and application

A technology of animal urine and urine, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the inconvenience of animal urine storage, transportation and on-site operation, less application of ELISA test paper cards and kits, quality control samples It can only be used and prepared immediately to achieve the effect of overcoming bad preservation, simple production process and easy preservation

Active Publication Date: 2016-08-17
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because animal urine is not convenient for storage, transportation and on-site operation, quality control samples can only be used and prepared immediately, so it is rarely used in ELISA test strips and kits

Method used

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  • A kind of animal urine dry powder for quality control of clenbuterol detection and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1, Preparation of Negative Pig Urine Dry Powder for Quality Control of Clenbuterol Detection

[0033] 1. Collection of pig urine

[0034] 1. In the experimental pig farm, select growing pigs in good health and who have not eaten clenbuterol (Du Dachang Sanyuan hybrid pigs), and place them in metabolic cages for pre-feeding for 7 days to adapt to the environment;

[0035] 2. After the pre-feeding period, collect the fresh urine excreted by the pigs in a natural state. The interval between the time of collecting pig urine and the time of pig urination should not exceed 10 minutes to ensure that the urine is fresh. Immediately place the collected fresh urine in a centrifuge at 1500×g for 15 minutes, collect the supernatant and store it temporarily in a refrigerator at 2-6°C;

[0036] 3. Mix the collected and purified urine by centrifugation every day, divide it into 1000mL plastic bottles, put it in the freezer, and store it below -18°C.

[0037] 2. Preparation o...

Embodiment 2

[0043] Example 2, Preparation of Positive Pig Urine Dry Powder for Quality Control of Clenbuterol Detection

[0044] 1. Collection of pig urine

[0045] 1. In the experimental pig farm, select growing pigs in good health and who have not eaten clenbuterol (Du Dachang Sanyuan hybrid pigs), and place them in metabolic cages for pre-feeding for 7 days to adapt to the environment;

[0046] 2. After the pre-feeding period, collect the fresh urine excreted by the pigs in a natural state, and the interval between the time of collecting pig urine and the time of pig urination should not exceed 10 minutes. The urine collected after each pig urination was immediately placed in a centrifuge at 1500×g for 15 minutes, and the supernatant was collected and temporarily stored in a refrigerator at 2-6°C;

[0047] 3. Mix the collected and purified urine by centrifugation every day, divide it into 1000mL plastic bottles, put it in the freezer, and store it below -18°C.

[0048] 2. Preparation...

Embodiment 3

[0057] Example 3, Application of Pig Urine Dry Powder for Quality Control of Clenbuterol Detection on Colloidal Gold Test Strips

[0058] 1. Preparation of pig urine dry powder recovery solution

[0059] Take one tube of negative pig urine dry powder and one positive pig urine dry powder, inject 1mL of pure water into two urine dry powder bottles with a syringe, shake fully to dissolve the dry powder completely, and form clenbuterol negative and positive pig urine reconstitution solution .

[0060] 2. Sampling inspection of test strips

[0061] Before the clenbuterol colloidal gold rapid detection test paper card (pig urine) is opened and used, 2 test paper cards are randomly taken out of a box (50 pieces / box). According to the conditions and operating steps required by the test paper card operating instructions, use a pipette to draw the negative and positive pig urine recovery solutions and drop them into the two test paper card sampling tanks respectively, and read the re...

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Abstract

The invention provides animal urine dry powder for detecting and controlling quality of clenbuterol as well as a preparation method and application of the animal urine dry powder. The animal urine dry powder comprises independently packaged feminine and masculine urine dry powder. The preparation method comprises the following steps: acquiring fresh urine, centrifuging, and collecting supernatant; performing vacuum drying at room temperature to obtain the feminine urine dry powder; collecting fresh urine, centrifuging, and collecting supernatant; adding a clenbuterol standard product, and mixing uniformly; and performing vacuum drying at room temperature to obtain the masculine urine dry powder. The animal urine dry powder can be used as a quality control sample used for clenbuterol enzyme-linked immunosorbent test paper cards and kits. The animal urine dry powder provided by the invention can be stored for a long time hermetically at normal temperature; after an appropriate amount of pure water is added into the dry powder, the dry powder is dissolved to form a solution which is the same as quality control samples of the clenbuterol enzyme-linked immunosorbent test paper cards and kits prepared by using fresh animal urine in application; and the animal urine dry powder can replace a clenbuterol detection quality control sample prepared from the fresh animal urine.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a dry animal urine powder for quality control of clenbuterol detection and its preparation method and application. Background technique [0002] Clenbuterol is the most important "clenbuterol" substance. There have been many incidents of clenbuterol poisoning caused by eating animal-derived foods at home and abroad. The Chinese government has explicitly banned the use of Clenbuterol in animal production. Tro. However, because clenbuterol has the effect of increasing the growth rate and lean meat rate of animals, there are still a few criminals who take risks and illegally manufacture, sell and use clenbuterol, resulting in clenbuterol being banned in animal production , resulting in frequent food safety incidents. Because clenbuterol is mainly used in the animal breeding stage, most of it is excreted in the urine in the form of the prototype drug. Therefore, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/558
CPCG01N33/535G01N33/558
Inventor 苏晓鸥王瑞国程劼王培龙樊霞李阳张维
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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