Method for analyzing tandem mass spectrometry driven by direct current voltage in ion trap mass analyzer

Abstract

The invention belongs to the technical field of mass spectrometry analysis and particularly relates to a method for analyzing tandem mass spectrometry driven by direct current voltage in an ion trap mass analyzer. The method specifically comprises the three stages of ion selection isolation, collision induction dissociation and mass analysis, wherein in the stage of collision induction dissociation, bias direct current voltage is generated in the center of an ion trap by exerting asymmetric waveform radio frequency working voltage on electrodes of the ion trap mass analyzer; by means of the bias direct current voltage, isolated parent ions with the certain mass-to-charge ratio are made to deviate from the constraint center of the ion trap to obtain energy so as to be excited, ions excited to a high-energy state can collide with neutral molecules in the ion trap and are dissociated, and thus tandem mass spectrometry analysis is achieved. The method for analyzing the tandem mass spectrometry driven by the direct current voltage in the ion trap mass analyzer has the advantages that an additional direct current main is not needed, the bias direct current voltage can be achieved only through control of software, time-sequence control is achieved, and an experimental device and method can be obviously simplified.

Description

technical field [0001] The invention belongs to the technical field of mass analysis, and in particular relates to a method for realizing cascaded mass spectrometry analysis in an ion trap mass spectrometer, which implements ion confinement and collision-induced dissociation by applying a radio frequency operating voltage with an asymmetric waveform. Background technique [0002] Mass spectrometry is a scientific technology that can accurately measure the weight of atoms and molecules. It is one of the most widely used analytical techniques and has been used in life sciences, food safety, environmental testing, national defense, pharmaceuticals, chemicals and other fields. role is becoming more and more important. As we all know, an important feature of a mass spectrometer is the tandem mass spectrometry function, which can characterize and analyze the structure of compounds through tandem mass spectrometry. The analysis process of tandem mass spectrometry is as follows: th...

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Problems solved by technology

However, this method requires an additional DC power supply to provide DC voltage. The dipole DC application method is realized through the modification of the circuit. At the same time, the dipole DC voltage has timing changes and requires precise control, so the hardware part of the instrument Requirements are bound to become more stringent and complex

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