A kind of wheat prolamin and glutenin and its preparation method and application
A wheat prolamin and glutenin technology, which is applied in the field of cereal active protein processing, can solve the problems of high cost and complicated production process, and achieve the effect of convenient compounding
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Embodiment 1
[0042] Embodiment 1: basic preparation method example
[0043] A preparation method of wheat prolamin and gluten, comprising the following steps:
[0044] (1) Weigh 1000g raw wheat flour, add alkaline alcohol solution (85% ethanol + 0.4% NaOH, mixed by volume ratio 55:45) according to 12:1 liquid-to-material ratio, extract at 45°C for 2h, settle and separate, Obtain supernatant 1;
[0045] (2) Concentrate the supernatant 1 at 50°C and an absolute pressure of 5.3 Kpa for 20 minutes until the volume is 1 / 3 of the original volume to obtain the concentrated solution 1;
[0046] (3) Adjust the pH value of the concentrated solution 1 to 6.8 with 2mol / L HCl, settle and separate, wash with water 3-5 times until neutral, and obtain the precipitate 1;
[0047] (4) Add 70% ethanol to the precipitate 1 according to the ratio of liquid to material of 10:1, stir and soak at room temperature for 2 hours, settle and separate to obtain the supernatant 2 and precipitate 2, wash the precipitat...
Embodiment 2
[0053] Embodiment 2: The influence of extraction solvent on wheat prolamin and glutenin yield
[0054] Wheat prolamin and glutenin were prepared by the method of Example 1, but the alkaline alcohol solution in step (1) was designed into six combinations as shown in Table 2. It can be known from Table 2 that when 0.4% NaOH solution and 85% ethanol are mixed as the extraction solution in a volume ratio of 45:55, the yield of wheat prolamin and glutenin is relatively highest, and the yield of gliadin is 36.5%. The yield of glutenin is 35.3%. If 0.4% NaOH solution and 85% isopropanol are mixed at a volume ratio of 45:55 as the extraction solution, the extraction efficiency is second only to the former. Comprehensive comparison shows that the composition and ratio of alkaline alcohol solution have a great influence on the yield of wheat prolamin and glutenin, but the yield is not high, which may be related to the interaction between gluten protein and sugar and other substances.
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Embodiment 3
[0057] Example 3: Effect of Concentration Conditions on the Elasticity and Extensibility of Wheat Prolamins and Glutenins
[0058] Wheat prolamin and gluten were prepared by the method of Example 1, but the concentration conditions in step (2) were designed as six schemes as shown in Table 3. It can be seen from Table 3 that with the increase of the concentration temperature and the extension of the concentration time, the elasticity and extensibility of the extracted gliadin and glutenin are reduced, and short-term concentration at a lower temperature is beneficial to keep the glutenin as much as possible. The extensibility and elasticity of gliadin and glutenin, but the purpose of concentration is not well achieved when concentrated at low temperature for a short time, and the volume of the concentrated solution is still large, which is not conducive to subsequent further extraction operations. Considering comprehensively, the optimal concentration conditions are concentrati...
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