Gene ahpmm related to vitamin C synthesis in peanut and its application

A technology for vitamins and peanuts, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of functional research and transgene regulation and report gaps, and achieve the effect of improving salt tolerance and increasing vitamin C content in leaves

Active Publication Date: 2017-08-18
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the PMM gene has been cloned in Arabidopsis thaliana, tobacco, soybean, rice, tomato, wheat, etc., but there is no report of cloning in peanut.

Method used

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  • Gene ahpmm related to vitamin C synthesis in peanut and its application
  • Gene ahpmm related to vitamin C synthesis in peanut and its application
  • Gene ahpmm related to vitamin C synthesis in peanut and its application

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Embodiment 1

[0031] Example 1: A peanut vitamin C synthesis-related gene AhPMM and its application in improving vitamin C content and salt tolerance

[0032] 1. Experimental materials

[0033] 1. Genes, strains and peanut varieties

[0034] The invention clones the peanut PMM gene (AhPMM), the Escherichia coli DH5α, and the Agrobacterium tumefaciens strain EHA105 are preserved in the laboratory of the Genetic Research Laboratory of Qingdao Agricultural University, and the engineered strains commercialized by biological companies can also be used. The transgenic recipient material is the peanut variety "Junan Xiaohongzhong" (provided by the Laboratory of Genetics Research Laboratory, Qingdao Agricultural University).

[0035] The sequence list of the cloned peanut AhPMM gene is shown in SEQ ID No: 1, and the amino acid sequence of the encoded protein is shown in SEQ ID No: 2.

[0036] The primer names and sequences for cloning the peanut AhPMM gene are as follows:

[0037] P1 (5'-AAATGGCTGCCCGGAAACCT...

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Abstract

The invention provides a peanut vitamin C synthesis related gene Ah PMM and an application thereof. A transgenic plant of which the total vitamin C and reduction state vitamin C (AsA) content is significantly improved can be obtained after the gene is subjected to over-expression in peanuts. Proved by experiments, the vitamin C content of peanut leaves can be significantly improved by performing over-expression on the Ah PMM gene provided by the invention without causing significant influences to normal growth of the peanuts. Protein and an encoding gene thereof provided by the invention have important theoretical and practical significance in research of synthesis mechanisms of plant vitamin C, improvement for increasing the plant vitamin C content and stress resistance, and are wide in application prospect.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to the peanut vitamin C synthesis-related gene AhPMM and its application. Background technique [0002] Vitamin C is ascorbic acid, which plays a regulatory role in the oxidation-reduction metabolism of organisms. Human lack of it can cause scurvy. In addition, it also has other functions. For example, lack of vitamin C cholesterol cannot be hydroxylated into bile acid and excreted from the body; its oxidase will affect the metabolism of certain amino acids (usually aromatic); and detoxification. Generally, vitamin C can be synthesized by itself in animals and plants, but ascorbic acid cannot be synthesized in humans. This is due to the lack of gulonolactone oxidase in the human body. Vitamin C synthesized by the plant itself can not only be used by humans, but also plays an important role in the physiological functions of the plant itself. Generally speaking, ascorbic acid is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/61C12N9/90A01H5/00
Inventor 隋炯明郑春花王亚赵春梅孔祥远王晶珊乔利仙
Owner QINGDAO AGRI UNIV
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