15 results about "Vitamin synthesis" patented technology

The invention relates to the technical field of foliar fertilizers, in particular to a sulfur-rich organic matter-containing foliar fertilizer additive for improving the nutritional value of fruits and vegetables. The foliar fertilizer additive containing the sulfur-rich organic matter and used for improving the nutritional value of the fruits and vegetables comprises the following raw materials in parts by weight: 20-30 parts of the sulfur-rich organic matter, 5-10 parts of tea polyphenol, 4-7 parts of a growth regulator, 10-14 parts of a slow release agent, 15-18 parts of bentonite and 4-8 parts of a seaweed extract. The sulfur-rich organic matter serves as a core component, the additive aims to provide necessary sulfur-containing amino acid for plants, and the amino acid plays an important role in various physiological processes in the plants and participates in protein and vitamin synthesis. By combining the tea polyphenol, the growth regulator, the slow-release agent and other functional components, the health state of plants can be comprehensively optimized, the content of amino acids and vitamins in fruits and vegetables is increased, and finally the purpose of increasing the nutritional value of the fruits and vegetables is achieved.

The invention relates to the technical field of functional foods, in particular to a fermented cereal formula food for controlling sugar and a preparation method thereof, three strains synergistically ferment cereals, and are compounded with a high-activity white hyacinth bean alpha-amylase inhibitor, high-purity mulberry leaf superfine powder and chromium-enriched yeast to jointly construct a multi-way blood sugar regulation and control system; according to the formula, three proteins of soybeans, whey and peas are combined with dietary fibers and prebiotics, the nutritional structure is optimized, satiety is enhanced, traditional herbal essences such as bitter gourds and Chinese yams are supplemented, the dual goals of scientific sugar control and nutrition balance are achieved through precise proportioning, deep biotransformation of cereal nutritional ingredients is achieved through a three-strain staged synergistic fermentation technology, and the nutritional value of the whole grain is improved. According to the present invention, the deep biological conversion of cereal nutritional components is achieved through the protein hydrolysis and glycoside hydrolysis ability of the lactobacillus plantarum, the glutamic acid decarboxylation ability of the bifidobacterium breve, and the vitamin synthesis and chromium enrichment ability of the saccharomyces cerevisiae;

The application provides a lighting control method, a lighting system and a terminal for a weak window space, and the method comprises the following steps: based on a preset space use rule, dynamically dividing a weak window space into a plurality of functional areas; the functional areas comprise a normal activity area, a transition guide area and a sunlight coordination area; acquiring a time period in which effective natural light exposure can be performed in the future, and defining the time period as a coordination time window; in the coordination time window, activating a lighting unit of the transition guide area to generate a dynamic light path connecting the normal activity area and the sunlight coordination area; the dynamic light path is used to guide a user to move from the normal activity area to the sunlight coordination area to receive natural light, so as to receive natural light meeting a vitamin D synthesis light dose threshold. The application significantly improves the biological availability and utilization efficiency of natural light resources in a weak window environment, and provides a feasible technical path for a healthy building, an underground space and a high-density urban office scene.

The invention relates to the technical field of gene engineering and enzyme engineering, in particular to peroxidase and a preparation method and application thereof.The peroxidase has high position specificity to C25-site hydroxylation of vitamin D3 and high tolerance to high-concentration H2O2, heterologous expression of the peroxidase in pichia pastoris can be achieved through the gene engineering technology, and the peroxidase has the advantages of being high in stability, good in stability and good in stability. And high-concentration vitamins are successfully and efficiently catalyzed to synthesize the 25-hydroxyvitamin D3.

The application discloses a cucumber CsGDH protein and application of a coding gene thereof in regulation and control of vitamin C synthesis, and an amino acid sequence of the CsGDH protein is shown as SEQ ID NO. 2. The application discloses a gene sequence of cucumber L-galactose dehydrogenase CsGDH and a coding protein thereof for the first time, and verifies a key function of the CsGDH in vitamin C synthesis, thereby providing a new gene resource for research on regulation and control of vitamin C metabolism.

A lipid-vitamin conjugate based on an amino acid structure and a synthesis method therefor. The head of the lipid-vitamin conjugate consists of a single amino acid or a plurality of amino acids, and the tail thereof consists of a hydrophobic vitamin and a fatty chain. The molecular weight of the amino acid-based lipid-vitamin conjugate is 1000-2000 Da. The synthesis method is a solid-phase synthesis method based on polypeptide synthesis, and the method comprises: first attaching the carbon terminus of an amino acid to a 2-chlorotrityl chloride resin, then carrying out condensation-washing-deprotection-washing cycle synthesis, and finally, coupling a vitamin and a fatty chain to the nitrogen terminus to obtain a lipid-vitamin conjugate based on an amino acid structure. The synthesized lipid-vitamin conjugate is cleaved from the resin by means of a cleavage solution, and soluble by-products are removed from the organic solvent by means of a non-polar solvent. The method features ease of operation, high product purity, minimal complex factors requiring control, and high reproducibility.

The invention provides electrolyte-containing coffee as well as a preparation method and application thereof, and belongs to the technical field of coffee beverages. According to the invention, only components such as coffee concentrate, instant coffee powder, erythritol, potassium citrate, sodium citrate, vitamin B3, vitamin B6, synthetic vitamin E acetate dry powder, grapefruit concentrated juice, purified water and the like are adopted, and preservatives or additives such as dipotassium phosphate and the like are not used. The black coffee liquid, the electrolyte and the grapefruit concentrated juice are effectively combined, so that absorption of intestinal tracts to lipid can be inhibited, generation of stimulated bile acid is reduced, the risk of gastric mucosa damage is reduced, the content of triglyceride and cholesterol in excrement is increased, accumulation of fat in bodies is reduced, and weight rise of high-fat diet mice can be effectively relieved.

This invention discloses a class of lipid vitamins based on amino acid structures and their synthesis method. The head of the lipid vitamin consists of one or more amino acids, and the tail consists of a hydrophobic vitamin and a fatty acid chain. The molecular weight of the amino acid lipid vitamin is 1000-2000 Da. The synthesis method is a solid-phase synthesis method based on peptide synthesis. First, the carbon terminus of the amino acid is linked to a 2-chlorotriphenylmethyl chloride resin. Then, a condensation-washing-deprotection-washing cycle is performed. Finally, the vitamin and fatty acid chain are coupled at the nitrogen terminus to obtain a class of lipid vitamins based on amino acid structures. The synthesized lipid vitamin is removed from the resin using a lysis buffer, and soluble byproducts in the organic solvent are removed using a non-polar solvent. This method is simple to operate, produces high-purity products, requires fewer complex control factors, and has strong reproducibility.

The application provides a light guide method and system for inducing vitamin D synthesis, a terminal and a medium, the method comprising: evaluating a sunlight deficiency risk level to which a user belongs; generating a multi-channel spectrum intervention strategy corresponding to the risk level; predicting a time period during which natural sunlight can be coordinated in the future, and defining the time period as a coordination time window; triggering a behavior guide event if the current time is within the coordination time window; the behavior guide event prompting the user to accept intervention light output by the multi-channel spectrum intervention strategy by generating a dynamic light guide path; the spectrum of the intervention light is in a non-ultraviolet light band, and is used for inducing vitamin D synthesis in the body. Under the premise of ensuring safety and comfort, the application uses light signals of specific non-ultraviolet bands to regulate related photosensitive pathways, thereby indirectly promoting the synthesis efficiency of vitamin D in the body.

The invention discloses a method for constructing a vitamin E production strain by synchronously editing and recombining three sites of a yeast genome and application, and relates to the technical field of synthetic biology and metabolic engineering. According to the invention, a helper plasmid for expressing CRISPR-Cas9 protein and three targeted specific site guide RNA (sgRNA) is constructed, and the helper plasmid and three vitamin E pathway gene donor fragments which are assembled and cloned in escherichia coli in advance are co-transformed into saccharomyces cerevisiae, so that efficient editing and large-scale gene fragment integration can be simultaneously carried out on three chromosome sites of HO, GAL80 and ARO10. The three-site synchronous editing and recombination efficiency of the method exceeds 80%. By utilizing the method, an engineering strain of a complete vitamin E synthesis route can be rapidly constructed at one time, and fermentation verifies that the yield of the vitamin E can reach 5.9 g / L. The invention provides an efficient and universal tool for quickly constructing complex metabolic pathways in yeast.

This invention relates to the field of foliar fertilizer technology, and more particularly to a foliar fertilizer additive containing sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables. The foliar fertilizer additive provided by this invention, containing sulfur-rich organic matter for enhancing the nutritional value of fruits and vegetables, comprises the following raw materials in parts by weight: 20-30 parts sulfur-rich organic matter, 5-10 parts tea polyphenols, 4-7 parts plant growth regulators, 10-14 parts slow-release agents, 15-18 parts bentonite, and 4-8 parts seaweed extract. With sulfur-rich organic matter as the core component, this additive aims to provide plants with essential sulfur-containing amino acids, which play important roles in various physiological processes within plants, participating in protein and vitamin synthesis. Combined with multiple functional components such as tea polyphenols, plant growth regulators, and slow-release agents, it can comprehensively optimize plant health, increase the amino acid and vitamin content in fruits and vegetables, and ultimately achieve the goal of enhancing the nutritional value of fruits and vegetables.

The invention discloses bacillus subtilis chassis cells for vitamin B6 production and a construction method of the bacillus subtilis chassis cells, and belongs to the technical field of bioengineering. The chassis cell is obtained by performing systematic knockout on eight genes including upp, srf, spo, aprE, nprE, nprB, mpr and wprA in bacillus subtilis 168 through a gene engineering technology, wherein the eight genes include the upp gene, the srf gene, the spo gene, the aprE gene, the nprE gene, the nprB gene, the mpr gene and the wprA gene. Precise knockout of the gene is realized by adopting a homologous recombination method, and the obtained chassis cell shows remarkably optimized physiological characteristics: the growth rate and the glucose metabolism rate are remarkably improved, and meanwhile, the sporulation rate and the extracellular protease activity are remarkably reduced. Experimental results show that the modified chassis cell has excellent suitability with a heterologous gene element of a vitamin B6 synthesis pathway, and can efficiently synthesize vitamin B6. Therefore, the recombinant bacillus subtilis chassis cell provided by the invention has important application value in the field of microbiological manufacturing of vitamin B6.

The present application belongs to the technical field of ultraviolet light therapy, and particularly relates to a method for screening UVB lamp beads and improving vitamin D synthesis, which comprises the following steps: parameter selection of the UVB lamp beads, wherein the parameters of the UVB lamp beads are divided into key parameters, main parameters and general parameters; inspection standards of the UVB lamp beads, wherein the inspection standards comprise inspection equipment, inspection equipment calibration, inspection operation process and inspection error analysis; setting of the UVB lamp bead matrix, wherein the number of the lamp beads is adjusted according to the size of the area light source, and the matrix arrangement is adjusted according to the driving current and the light chamber; denoising of the UVB spectrum, wherein the background light removal method is adopted to determine the effective wavelength range of the spectrum; and calculation of the vitamin D synthesis efficiency, wherein the efficiency is calculated based on the effective wavelength range of the UVB spectrum and the vitamin D synthesis efficiency model.

This invention relates to the field of risk assessment technology, specifically to a big data-based method and system for osteoporosis risk assessment. The method includes the following steps: acquiring daily UVB intensity at various times and accumulating it with exposure duration to determine vitamin D synthesis conditions; statistically analyzing static work type and total exposure time to determine insufficient bone load stimulation; combining bone density decline to determine the risk of bone stress pathway degeneration; assessing whether annual weight changes disrupt bone support; and integrating the results to generate an osteoporosis risk level label. In this invention, by introducing the cumulative calculation of hourly UVB irradiation intensity and exposure time in an individual's residential area, it determines whether vitamin D synthesis meets the effective conditions required for bone metabolism. Combining static work behavior labels and total static work time, it identifies insufficient bone load conditions. Furthermore, it performs trend analysis on the decreasing changes in distal tibial bone density over consecutive weeks, achieving dynamic identification of bone stress pathway degeneration and improving the timeliness and reliability of risk assessment.

The invention discloses a wearable vitamin D intelligent synthesis physiotherapy instrument based on phototherapy, and belongs to the field of biomedicine. A wearable vitamin D intelligent synthesis physiotherapy instrument based on phototherapy comprises a wearable main body, a data sensing unit, an intelligent control unit and a safety protection unit. The problems that an existing vitamin D supplementing mode is low in absorption efficiency and large in individual difference are solved, continuous vitamin D synthesis support is provided for a user through cooperation of the wearable body and the phototherapy assembly, physiological parameters such as the vitamin D content, the skin temperature and the heart rate can be monitored in real time through the sensor array, and the user experience is improved. The method provides an accurate basis for phototherapy, ensures maximization of the phototherapy effect, dynamically adjusts the phototherapy time, intensity and frequency according to monitoring data, realizes a personalized treatment scheme, adapts to different user requirements, sets a safety threshold, automatically pauses phototherapy once the monitoring data is abnormal, and ensures the use safety of the user.