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Application of Noggin gene as exogenous gene introduced into cashmere goat cells to improve villus fineness

A technology of exogenous genes and cashmere goats, applied in the direction of plant gene improvement, application, genetic engineering, etc.

Inactive Publication Date: 2015-02-25
LIAONING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Those skilled in the art know that exogenous genes are DNA sequences introduced into target cells through genetic engineering techniques or virus infection, but so far, there is no information about using Noggin gene as exogenous genes through genetic engineering techniques or viral infections. Reports on introducing cashmere goat cells to improve the fineness of cashmere

Method used

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  • Application of Noggin gene as exogenous gene introduced into cashmere goat cells to improve villus fineness
  • Application of Noggin gene as exogenous gene introduced into cashmere goat cells to improve villus fineness
  • Application of Noggin gene as exogenous gene introduced into cashmere goat cells to improve villus fineness

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Experimental program
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Embodiment Construction

[0011] 1. Primary Skin Cell Culture of Liaoning Cashmere Goat

[0012] (1) Select about 5cm from the buttocks of Liaoning cashmere goats 2 Use wool shears to cut off the long hair and velvet, disinfect with iodine, and then use 75% alcohol to remove the code, and cut about 0.5cm with a blade. 3 Immediately put tissue pieces of the same size into 75% alcohol, wash for 30s, rinse three times with saline containing 100U / ml double antibody, and finally put sheepskin tissue pieces into D-Hanks, seal with parafilm, and store at 4°C Save and bring back to the laboratory within 4 hours.

[0013] (2) Place the small piece of sheep skin in D-Hanks buffer, rinse it three times with D-Hanks solution, and then cut it into 1mm with ophthalmic scissors 3 Small tissue blocks left and right. Take small pieces of tissue and place them in the culture bottle and evenly distribute them at the bottom of the culture bottle. After the left and right sides are completed, the bottom of the bottle ...

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Abstract

The invention discovers that a Noggin gene generates a regulation and control effect on the villus fineness of Liaoning cashmere goat, that is to say, the higher expression quantity of the Noggin gene can promote the expression quantity of KAP7.1 and KAP8.2 genes to be increased, the increase of the genes can reduce the villus fineness and improve the villus quality, and thus an application of the Noggin gene as an exogenous gene introduced into cashmere goat cells to improve the villus fineness is disclosed; Noggin gene over-expressed lentivirus is injected into a male Liaoning cashmere goat testis, and after the KAP26.1 gene as an exogenous gene is introduced into the cashmere goat cells through virus injection, a Liaoning cashmere goat individual having high expression quantity of the KAP7.1 and KAP8.2 genes is selected and bred as a breeding sheep, so that the villus fineness of the cashmere goat can be improved, and the villus quality is increased.

Description

technical field [0001] The invention relates to a new application of Noggin gene, in particular to the application of Noggin gene as an exogenous gene introduced into cashmere goat cells to improve the fineness of cashmere. Background technique [0002] Cashmere goat hair consists of coarse hairs produced by primary hair follicles and down hairs produced by secondary hair follicles. Velvet has slenderness, lightness, smoothness, warmth, soft luster, high elasticity, excellent textile performance and other characteristics incomparable to other fibers. It is the most precious natural fiber raw material produced by the world's textile industry and is known as "soft gold". Improving the quality of fluff and increasing the output of fluff are the two main indicators to improve the economic value of cashmere goats, and the fineness of fluff is the main index to measure the quality of fluff, that is, the finer the fluff, the better the quality. In 1996, Zimmerman LB found that Nog...

Claims

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Application Information

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IPC IPC(8): C12N15/867A01K67/027
Inventor 金梅赵凤琴张丽丽
Owner LIAONING NORMAL UNIVERSITY
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