Device for measuring delayed fluorescence decay of algae
A delayed fluorescence, algae technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of complex detection process, slow speed, large consumption of algae inhibitor, etc., to achieve good repeatability, low consumption, and detection speed. quick effect
Inactive Publication Date: 2015-02-25
CHONGQING UNIV
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Problems solved by technology
[0003] The technical problem to be solved by the present invention is to provide a device for measuring the delayed fluorescence decay of algae, which can avoid the influence of dead algae cells during detection, and accurately characterize the degree of inhibition of algae growth by algae inhibitors. In the technology, the detection process is complicated, the speed is slow, and the consumption of algicide is large, etc.
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[0010] Below in conjunction with accompanying drawing and embodiment the present invention will be further described:
[0011] Such as figure 1 As shown, the present invention includes a semi-flowing algae culture medium 1, an algastatic agent injector 3, an LED red light 4, a quartz excitation pool 5, a quartz reaction pool 8, a fluorescence detection spectrometer 16 and an integrator 17, placed in a common glass bottle The semi-flowing algae medium 1 in the medium is connected to the three-way valve 9 through the first miniature peristaltic pump 2, and the three-way valve 9 is connected to the quartz excitation pool 5 on the one hand, and on the other hand to the algicide injector 3, and the quartz excitation pool 5 passes through The optical fiber is connected to the LED red light 4, the quartz excitation cell 5 is connected to the quartz reaction cell 8 through the second miniature peristaltic pump 6, the quartz reaction cell 8 is connected to the fluorescence spectrum...
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The invention discloses a device for measuring delayed fluorescence decay of algae. The device is structurally characterized in that a semi-flowing alga culture medium (1) is connected with a three-way valve (9) by virtue of a first micro peristaltic pump (2), the three-way valve (9) is communicated with a quartz excitation tank (5) on one hand and is connected to an alga inhibiting agent injector (3) on the other hand, the quartz excitation tank (5) is connected with an LED red light lamp (4) by virtue of an optical fiber and is connected with a quartz reaction tank (8) by virtue of a second micro peristaltic pump (6), and the quartz reaction tank (8) is connected with a fluorescence spectrum detector (10) by virtue of an optical fiber. The device disclosed by the invention can be used for continuously detecting the fluorescence decay of blank alga samples and alga samples with an alga inhibiting agent by virtue of the alga inhibiting agent injector, and can be used for characterizing the influence degree of the alga inhibiting agent on floating algae by virtue of integrals of attenuation of photons emitted within 90 seconds. According to the device disclosed by the invention, delayed fluorescence for characterizing alga growth inhibition can be rapidly measured, the precision and reproducibility of experiments can be improved, and the consumption of the alga inhibiting agent can be saved.
Description
technical field [0001] The invention relates to a device for measuring substances, in particular to a device for measuring planktonic algae in water, in particular to a device for rapidly measuring the degree of algae being affected by an algastatic agent. Background technique [0002] The excessive discharge of sewage with high nitrogen and phosphorus content has led to the aggravation of eutrophication in water bodies, and the frequent outbreak of algae blooms has become a hot spot of global concern. The inhibition of phytoplankton growth is the key to solving the problem of water pollution. the key of. The birth of a small number of algae inhibitors has inhibited the growth of algae to a certain extent. At present, the detection of algae cell number and chlorophyll a can roughly characterize the degree to which algae are affected by algastatic agents. However, this detection process is complex, slow, and consumes a lot of medicaments. Contents of the invention [000...
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IPC IPC(8): G01N21/64
Inventor 马华张洁
Owner CHONGQING UNIV